1jhl
From Proteopedia
(New page: 200px<br /> <applet load="1jhl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jhl, resolution 2.4Å" /> '''THREE-DIMENSIONAL ST...) |
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| - | [[Image:1jhl. | + | [[Image:1jhl.jpg|left|200px]]<br /><applet load="1jhl" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1jhl, resolution 2.4Å" /> | caption="1jhl, resolution 2.4Å" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JHL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Phasianus_colchicus Phasianus colchicus]. Full crystallographic information is available from [http:// | + | 1JHL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Phasianus_colchicus Phasianus colchicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: complex(antibody-antigen)]] | [[Category: complex(antibody-antigen)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:07:33 2008'' |
Revision as of 14:07, 15 February 2008
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THREE-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX
Overview
Although antibodies are highly specific, cross-reactions are frequently, observed. To understand the molecular basis of this phenomenon, we studied, the anti-hen egg lysozyme (HEL) monoclonal antibody (mAb) D11.15, which, cross-reacts with several avian lysozymes, in some cases with a higher, affinity (heteroclitic binding) than for HEL. We have determined the, crystal structure of the Fv fragment of D11.15 complexed with pheasant egg, lysozyme (PHL). In addition, we have determined the structure of PHL, Guinea fowl egg lysozyme, and Japanese quail egg lysozyme. Differences in, the affinity of D11.15 for the lysozymes appear to result from sequence, substitutions in these antigens at the interface with the antibody. More, generally, cross-reactivity is seen to require a stereochemically, permissive environment for the variant antigen residues at the, antibody-antigen interface.
About this Structure
1JHL is a Protein complex structure of sequences from Mus musculus and Phasianus colchicus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex., Chitarra V, Alzari PM, Bentley GA, Bhat TN, Eisele JL, Houdusse A, Lescar J, Souchon H, Poljak RJ, Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7711-5. PMID:8356074
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