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2pfn

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[[Image:2pfn.gif|left|200px]]
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{{STRUCTURE_2pfn| PDB=2pfn | SCENE= }}
{{STRUCTURE_2pfn| PDB=2pfn | SCENE= }}
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'''Na in the active site of DNA Polymerase lambda'''
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===Na in the active site of DNA Polymerase lambda===
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==Overview==
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The incorporation of dNMPs into DNA by polymerases involves a phosphoryl transfer reaction hypothesized to require two divalent metal ions. Here we investigate this hypothesis using as a model human DNA polymerase lambda (Pol lambda), an enzyme suggested to be activated in vivo by manganese. We report the crystal structures of four complexes of human Pol lambda. In a 1.9 A structure of Pol lambda containing a 3'-OH and the non-hydrolyzable analog dUpnpp, a non-catalytic Na+ ion occupies the site for metal A and the ribose of the primer-terminal nucleotide is found in a conformation that positions the acceptor 3'-OH out of line with the alpha-phosphate and the bridging oxygen of the pyrophosphate leaving group. Soaking this crystal in MnCl2 yielded a 2.0 A structure with Mn2+ occupying the site for metal A. In the presence of Mn2+, the conformation of the ribose is C3'-endo and the 3'-oxygen is in line with the leaving oxygen, at a distance from the phosphorus atom of the alpha-phosphate (3.69 A) consistent with and supporting a catalytic mechanism involving two divalent metal ions. Finally, soaking with MnCl2 converted a pre-catalytic Pol lambda/Na+ complex with unreacted dCTP in the active site into a product complex via catalysis in the crystal. These data provide pre- and post-transition state information and outline in a single crystal the pathway for the phosphoryl transfer reaction carried out by DNA polymerases.
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{{ABSTRACT_PUBMED_17475573}}
==About this Structure==
==About this Structure==
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[[Category: Manganese]]
[[Category: Manganese]]
[[Category: Phosphoryl transfer reaction]]
[[Category: Phosphoryl transfer reaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:00:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:09:34 2008''

Revision as of 07:09, 29 July 2008

Image:2pfn.png

Template:STRUCTURE 2pfn

Na in the active site of DNA Polymerase lambda

Template:ABSTRACT PUBMED 17475573

About this Structure

2PFN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Role of the catalytic metal during polymerization by DNA polymerase lambda., Garcia-Diaz M, Bebenek K, Krahn JM, Pedersen LC, Kunkel TA, DNA Repair (Amst). 2007 Sep 1;6(9):1333-40. Epub 2007 May 1. PMID:17475573

Page seeded by OCA on Tue Jul 29 10:09:34 2008

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