1jtp
From Proteopedia
(New page: 200px<br /> <applet load="1jtp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jtp, resolution 1.9Å" /> '''Degenerate interface...) |
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caption="1jtp, resolution 1.9Å" /> | caption="1jtp, resolution 1.9Å" /> | ||
'''Degenerate interfaces in antigen-antibody complexes'''<br /> | '''Degenerate interfaces in antigen-antibody complexes'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In most of the work dealing with the analysis of protein-protein | + | In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics. |
==About this Structure== | ==About this Structure== | ||
| - | 1JTP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] with NA and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1JTP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Maes, D.]] | [[Category: Maes, D.]] | ||
[[Category: Muyldermans, S.]] | [[Category: Muyldermans, S.]] | ||
| - | [[Category: Transue, T | + | [[Category: Transue, T R.]] |
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: FMT]] | [[Category: FMT]] | ||
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[[Category: vhh]] | [[Category: vhh]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:38 2008'' |
Revision as of 11:26, 21 February 2008
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Degenerate interfaces in antigen-antibody complexes
Overview
In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.
About this Structure
1JTP is a Protein complex structure of sequences from Camelus dromedarius and Meleagris gallopavo with and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:11676532
Page seeded by OCA on Thu Feb 21 13:26:38 2008
Categories: Camelus dromedarius | Lysozyme | Meleagris gallopavo | Protein complex | Decanniere, K. | Desmyter, A. | Maes, D. | Muyldermans, S. | Transue, T R. | Wyns, L. | FMT | NA | Binding | Heavy chain antibody | Immunoglobulin | Interface | Vhh
