1jv5
From Proteopedia
(New page: 200px<br /> <applet load="1jv5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jv5, resolution 2.2Å" /> '''Anti-blood group A F...) |
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caption="1jv5, resolution 2.2Å" /> | caption="1jv5, resolution 2.2Å" /> | ||
'''Anti-blood group A Fv'''<br /> | '''Anti-blood group A Fv'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1JV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:09:41 2008'' |
Revision as of 14:09, 15 February 2008
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Anti-blood group A Fv
Overview
The specificity of antibody recognition of the ABO blood group, trisaccharide antigens has been explored by crystal structure analysis and, mutation methods. The crystal structure of the Fv corresponding to the, anti-blood group A antibody AC1001 has been determined to 2.2-A resolution, and reveals a binding pocket that is complementary to the blood group, A-trisaccharide antigen. The effect of mutating specific residues lining, this pocket on binding to the A and B blood group oligosaccharide antigens, was investigated through a panel of single point mutations and through a, phage library of mutations in complementarity determining region H3. Both, approaches gave several mutants with improved affinity for antigen., Surface plasmon resonance indicated up to 8-fold enhancement in affinity, for the A-pentasaccharide with no observable binding to the blood group B, antigen. This is the first example of single point mutations in a, carbohydrate-binding antibody resulting in significant increases in, binding affinity without loss of specificity.
About this Structure
1JV5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificity., Thomas R, Patenaude SI, MacKenzie CR, To R, Hirama T, Young NM, Evans SV, J Biol Chem. 2002 Jan 18;277(3):2059-64. Epub 2001 Oct 25. PMID:11679577
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