1kfa
From Proteopedia
(New page: 200px<br /> <applet load="1kfa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kfa, resolution 2.80Å" /> '''Crystal structure o...) |
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caption="1kfa, resolution 2.80Å" /> | caption="1kfa, resolution 2.80Å" /> | ||
'''Crystal structure of Fab fragment complexed with gibberellin A4'''<br /> | '''Crystal structure of Fab fragment complexed with gibberellin A4'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GA4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GA4:'>GA4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: immunoglobuiln fold]] | [[Category: immunoglobuiln fold]] | ||
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Revision as of 14:13, 15 February 2008
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Crystal structure of Fab fragment complexed with gibberellin A4
Overview
Gibberellins, a class of plant hormones, consist of more than 120 members., Only a few of them are recognized by a receptor that remains unknown. The, haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against, gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody, and GA(4). We carried out an X-ray crystallographic analysis of the, 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by, using the molecular replacement method. The crystal structure of the Fab, fragment showed the typical immunoglobulin fold of the beta-barrel, structure which is the common motif of all antibodies. A small, hapten-combining site was made up of three heavy chain CDR loops. On the, other hand, CDRs of the light chain did not interact directly with GA(4)., The C/D rings of the GA(4) molecule were in van der Waals contact mainly, with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3, beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
About this Structure
1KFA is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:12054627
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