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2pjx

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{{STRUCTURE_2pjx| PDB=2pjx | SCENE= }}
{{STRUCTURE_2pjx| PDB=2pjx | SCENE= }}
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'''Crystal structure of the Munc18c/Syntaxin4 N-peptide complex'''
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===Crystal structure of the Munc18c/Syntaxin4 N-peptide complex===
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==Overview==
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Sec1/Munc18 proteins (SM proteins) bind to soluble NSF attachment protein receptors (SNAREs) and play an essential role in membrane fusion. Divergent modes of regulation have been proposed for different SM proteins indicating that they can either promote or inhibit SNARE assembly. This is in part because of discrete modes of binding that have been described for various SM/SNARE complexes. One mode suggests that SM proteins bind only to Syntaxins (Stx) preventing SNARE assembly, whereas in another they facilitate SNARE assembly and bind to SNARE complexes. The mammalian cell surface SM protein Munc18c binds to an N-peptide in Stx4, and this is compatible with its interaction with SNARE complexes. Here we describe the crystal structure of Munc18c in complex with the Stx4 N-peptide. This structure shows remarkable similarity with a yeast complex indicating that the mode of binding, which can accommodate SNARE complexes, is highly conserved throughout evolution. Modeling reveals the presence of the N-peptide binding mode in most but not all yeast and mammalian SM/Stx pairs, suggesting that it has coevolved to fulfill a specific regulatory function. It is unlikely that the N-peptide interaction alone accounts for the specificity in SM/SNARE binding, implicating other contact surfaces in this function. Together with other data, our results support a sequential two-state model for SM/SNARE binding involving an initial interaction via the Stx N-peptide, which somehow facilitates a second, more comprehensive interaction comprising other contact surfaces in both proteins.
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==About this Structure==
==About this Structure==
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[[Category: Snare protein]]
[[Category: Snare protein]]
[[Category: Syntaxin]]
[[Category: Syntaxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:06:02 2008''

Revision as of 13:06, 29 July 2008

Image:2pjx.png

Template:STRUCTURE 2pjx

Crystal structure of the Munc18c/Syntaxin4 N-peptide complex

Template:ABSTRACT PUBMED 17517664

About this Structure

2PJX is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure of the Munc18c/Syntaxin4 N-peptide complex defines universal features of the N-peptide binding mode of Sec1/Munc18 proteins., Hu SH, Latham CF, Gee CL, James DE, Martin JL, Proc Natl Acad Sci U S A. 2007 May 22;104(21):8773-8. Epub 2007 May 16. PMID:17517664

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