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2pk2

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{{STRUCTURE_2pk2| PDB=2pk2 | SCENE= }}
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'''Cyclin box structure of the P-TEFb subunit Cyclin T1 derived from a fusion complex with EIAV Tat'''
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===Cyclin box structure of the P-TEFb subunit Cyclin T1 derived from a fusion complex with EIAV Tat===
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==Overview==
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The positive transcription elongation factor b (P-TEFb) is an essential regulator of viral gene expression during the life cycle of human immunodeficiency virus type 1 (HIV-1). Its cyclin T1 subunit forms a ternary complex with the viral transcriptional transactivator (Tat) protein and the transactivation response (TAR) RNA element thereby activating cyclin dependent kinase 9 (Cdk9), which stimulates transcription at the level of chain elongation. We report the structure of the cyclin box domain of human cyclin T1 at a resolution of 2.67 A. The structure was obtained by crystallographic analysis of a fusion protein composed of cyclin T1 linked to the transactivator protein Tat from equine infectious anemia virus (EIAV), which is functionally and structurally related to HIV-1 Tat. The conserved cyclin box domain of cyclin T1 exhibits structural features for interaction with physiological binding partners such as Cdk9. A recognition site for Cdk/Cyclin substrates is partly covered by a cyclin T-specific insert, suggesting specific interactions with regulatory factors. The previously identified Tat/TAR recognition motif (TRM) forms a C-terminal helix that is partly occluded in the cyclin box repeat interface, while cysteine 261 is accessible to form an intermolecular zinc finger with Tat. Residues of the TRM contribute to a positively charged groove that may directly attract RNA molecules. The EIAV Tat protein instead appeared undefined from the electron density map suggesting that it is highly disordered. Functional experiments confirmed the TAR binding properties of the fusion protein and suggested residues on the second cyclin box repeat to contribute to Tat stimulated transcription.
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{{ABSTRACT_PUBMED_17540406}}
==About this Structure==
==About this Structure==
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[[Category: Transcription regulation p-tefb]]
[[Category: Transcription regulation p-tefb]]
[[Category: Twinning]]
[[Category: Twinning]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:16:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:34:04 2008''

Revision as of 06:34, 29 July 2008

Image:2pk2.png

Template:STRUCTURE 2pk2

Cyclin box structure of the P-TEFb subunit Cyclin T1 derived from a fusion complex with EIAV Tat

Template:ABSTRACT PUBMED 17540406

About this Structure

2PK2 is a Single protein structure of sequence from Homo sapiens, equine infectious anemia virus. Full crystallographic information is available from OCA.

Reference

Cyclin box structure of the P-TEFb subunit cyclin T1 derived from a fusion complex with EIAV tat., Anand K, Schulte A, Fujinaga K, Scheffzek K, Geyer M, J Mol Biol. 2007 Jul 27;370(5):826-36. Epub 2007 May 10. PMID:17540406

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