1mfe
From Proteopedia
(New page: 200px<br /> <applet load="1mfe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mfe, resolution 2.0Å" /> '''RECOGNITION OF A CEL...) |
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| - | [[Image:1mfe.gif|left|200px]]<br /> | + | [[Image:1mfe.gif|left|200px]]<br /><applet load="1mfe" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1mfe" size=" | + | |
caption="1mfe, resolution 2.0Å" /> | caption="1mfe, resolution 2.0Å" /> | ||
'''RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT'''<br /> | '''RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.05 angstrom (A) resolution crystal structure of a | + | The 2.05 angstrom (A) resolution crystal structure of a dodecasaccharide-Fab complex revealed an unusual carbohydrate recognition site, defined by aromatic amino acids and a structured water molecule, rather than the carboxylic acid and amide side chains and a structured water molecule, rather than the carboxylic acid and amide side chains that are features of transport and other carbohydrate binding proteins. A trisaccharide epitope of a branched bacterial lipopolysaccharide fills this hydrophobic pocket (8 A deep by 7 A wide) in an entropy-assisted association (association constant = 2.05 x 10(5) liters per mole, enthalpy = -20.5 +/- 1.7 kilojoules per mole, and temperature times entropy = +10.0 +/- 2.9 kilojoules per mole). The requirement for the complementarity of van der Waals surfaces and the requirements of saccharide-saccharide and protein-saccharide hydrogen-bonding networks determine the antigen conformation adopted in the bound state. |
==About this Structure== | ==About this Structure== | ||
| - | 1MFE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1MFE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:42 2008'' |
Revision as of 11:54, 21 February 2008
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RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT
Overview
The 2.05 angstrom (A) resolution crystal structure of a dodecasaccharide-Fab complex revealed an unusual carbohydrate recognition site, defined by aromatic amino acids and a structured water molecule, rather than the carboxylic acid and amide side chains and a structured water molecule, rather than the carboxylic acid and amide side chains that are features of transport and other carbohydrate binding proteins. A trisaccharide epitope of a branched bacterial lipopolysaccharide fills this hydrophobic pocket (8 A deep by 7 A wide) in an entropy-assisted association (association constant = 2.05 x 10(5) liters per mole, enthalpy = -20.5 +/- 1.7 kilojoules per mole, and temperature times entropy = +10.0 +/- 2.9 kilojoules per mole). The requirement for the complementarity of van der Waals surfaces and the requirements of saccharide-saccharide and protein-saccharide hydrogen-bonding networks determine the antigen conformation adopted in the bound state.
About this Structure
1MFE is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Recognition of a cell-surface oligosaccharide of pathogenic Salmonella by an antibody Fab fragment., Cygler M, Rose DR, Bundle DR, Science. 1991 Jul 26;253(5018):442-5. PMID:1713710
Page seeded by OCA on Thu Feb 21 13:54:42 2008
