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2po4

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{{STRUCTURE_2po4| PDB=2po4 | SCENE= }}
{{STRUCTURE_2po4| PDB=2po4 | SCENE= }}
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'''X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase'''
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===X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase===
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==Overview==
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Coliphage N4 virion RNA polymerase (vRNAP), which is injected into the host upon infection, transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure. Here, we describe the 2.0-A resolution x-ray crystal structure of N4 mini-vRNAP, a member of the T7-like, single-unit RNAP family and the minimal component having all RNAP functions of the full-length vRNAP. The structure resembles a "fisted right hand" with Fingers, Palm and Thumb subdomains connected to an N-terminal domain. We established that the specificity loop extending from the Fingers along with W129 of the N-terminal domain play critical roles in hairpin-promoter recognition. A comparison with the structure of the T7 RNAP initiation complex reveals that the pathway of the DNA to the active site is blocked in the apo-form vRNAP, indicating that vRNAP must undergo a large-scale conformational change upon promoter DNA binding and explaining the highly restricted promoter specificity of vRNAP that is essential for phage early transcription.
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(as it appears on PubMed at http://www.pubmed.gov), where 18362338 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18362338}}
==About this Structure==
==About this Structure==
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[[Category: Right hand shape]]
[[Category: Right hand shape]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:31:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:56:26 2008''

Revision as of 01:56, 29 July 2008

Image:2po4.png

Template:STRUCTURE 2po4

X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase

Template:ABSTRACT PUBMED 18362338

About this Structure

2PO4 is a Single protein structure of sequence from Enterobacteria phage n4. Full crystallographic information is available from OCA.

Reference

X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase., Murakami KS, Davydova EK, Rothman-Denes LB, Proc Natl Acad Sci U S A. 2008 Mar 24;. PMID:18362338

Page seeded by OCA on Tue Jul 29 04:56:26 2008

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