2pox

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{{STRUCTURE_2pox| PDB=2pox | SCENE= }}
{{STRUCTURE_2pox| PDB=2pox | SCENE= }}
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'''Dark state structure of the reversibly switchable fluorescent protein Dronpa'''
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===Dark state structure of the reversibly switchable fluorescent protein Dronpa===
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==Overview==
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Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.
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(as it appears on PubMed at http://www.pubmed.gov), where 17646653 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17646653}}
==About this Structure==
==About this Structure==
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[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
[[Category: Reversibly switchable fluorescent protein]]
[[Category: Reversibly switchable fluorescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:33:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:07:01 2008''

Revision as of 04:07, 28 July 2008

Image:2pox.png

Template:STRUCTURE 2pox

Dark state structure of the reversibly switchable fluorescent protein Dronpa

Template:ABSTRACT PUBMED 17646653

About this Structure

2POX is a Single protein structure of sequence from Echinophyllia sp. sc22. Full crystallographic information is available from OCA.

Reference

Structural basis for reversible photoswitching in Dronpa., Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S, Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9. Epub 2007 Jul 23. PMID:17646653

Page seeded by OCA on Mon Jul 28 07:07:01 2008

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