This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ppz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ppz.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2ppz.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ppz| PDB=2ppz | SCENE= }}
{{STRUCTURE_2ppz| PDB=2ppz | SCENE= }}
-
'''NMR solution Structure of the Villin Headpiece Mutant G34L'''
+
===NMR solution Structure of the Villin Headpiece Mutant G34L===
-
==Overview==
+
<!--
-
BACKGROUND: As a rule, peptides are more flexible and unstructured than proteins with their substantial stabilizing hydrophobic cores. Nevertheless, a few stably folding peptides have been discovered. This raises the question whether there may be more such peptides that are unknown as yet. These molecules could be helpful in basic research and medicine. RESULTS: As a method to explore the space of conformationally stable peptides, we have developed an evolutionary algorithm that allows optimization of sequences with respect to several criteria simultaneously, for instance stability, accessibility of arbitrary parts of the peptide, etc. In a proof-of-concept experiment we have perturbed the sequence of the peptide Villin Headpiece, known to be stable in vitro. Starting from the perturbed sequence we applied our algorithm to optimize peptide stability and accessibility of a loop. Unexpectedly, two clusters of sequences were generated in this way that, according to our criteria, should form structures with higher stability than the wild-type. The structures in one of the clusters possess a fold that markedly differs from the native fold of Villin Headpiece. One of the mutants predicted to be stable was selected for synthesis, its molecular 3D-structure was characterized by nuclear magnetic resonance spectroscopy, and its stability was measured by circular dichroism. Predicted structure and stability were in good agreement with experiment. Eight other sequences and structures, including five with a non-native fold are provided as bona fide predictions. CONCLUSION: The results suggest that much more conformationally stable peptides may exist than are known so far, and that small fold classes could comprise well-separated sub-folds.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18284690}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18284690 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18284690}}
==About this Structure==
==About this Structure==
-
2PPZ is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PPZ OCA].
+
2PPZ is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PPZ OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Structural protein]]
[[Category: Structural protein]]
[[Category: Thermostable]]
[[Category: Thermostable]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:36:14 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:50:24 2008''

Revision as of 13:50, 28 July 2008

Image:2ppz.png

Template:STRUCTURE 2ppz

NMR solution Structure of the Villin Headpiece Mutant G34L

Template:ABSTRACT PUBMED 18284690

About this Structure

2PPZ is a Single protein structure. Full experimental information is available from OCA.

Reference

Evolutionary Pareto-optimization of stably folding peptides., Gronwald W, Hohm T, Hoffmann D, BMC Bioinformatics. 2008 Feb 19;9:109. PMID:18284690

Page seeded by OCA on Mon Jul 28 16:50:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ppz"
Personal tools