1ngx
From Proteopedia
(New page: 200px<br /> <applet load="1ngx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ngx, resolution 1.80Å" /> '''Chimeric Germline F...) |
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| - | [[Image:1ngx.gif|left|200px]]<br /> | + | [[Image:1ngx.gif|left|200px]]<br /><applet load="1ngx" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ngx" size=" | + | |
caption="1ngx, resolution 1.80Å" /> | caption="1ngx, resolution 1.80Å" /> | ||
'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''<br /> | '''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the Michaelis complex between the Fab fragment of | + | The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1NGX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with JEF as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NGX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=JEF:'>JEF</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Andryski, S | + | [[Category: Andryski, S E.]] |
| - | [[Category: Beuscher, A | + | [[Category: Beuscher, A B.]] |
| - | [[Category: Schultz, P | + | [[Category: Schultz, P G.]] |
| - | [[Category: Stevens, R | + | [[Category: Stevens, R C.]] |
[[Category: Yin, J.]] | [[Category: Yin, J.]] | ||
[[Category: JEF]] | [[Category: JEF]] | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:56 2008'' |
Revision as of 12:05, 21 February 2008
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Chimeric Germline Fab 7g12 with jeffamine fragment bound
Overview
The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.
About this Structure
1NGX is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112 [[Category: ]]
Page seeded by OCA on Thu Feb 21 14:05:56 2008
