2pse

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{{STRUCTURE_2pse| PDB=2pse | SCENE= }}
{{STRUCTURE_2pse| PDB=2pse | SCENE= }}
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'''Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis'''
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===Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis===
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==Overview==
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Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.
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(as it appears on PubMed at http://www.pubmed.gov), where 17980388 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17980388}}
==About this Structure==
==About this Structure==
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[[Category: Luciferase]]
[[Category: Luciferase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:43:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:05:45 2008''

Revision as of 00:05, 29 July 2008

Image:2pse.png

Template:STRUCTURE 2pse

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Template:ABSTRACT PUBMED 17980388

About this Structure

2PSE is a Single protein structure of sequence from Renilla reniformis. Full crystallographic information is available from OCA.

Reference

Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis., Loening AM, Fenn TD, Gambhir SS, J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:17980388

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