1pz5
From Proteopedia
(New page: 200px<br /> <applet load="1pz5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pz5, resolution 1.80Å" /> '''Structural basis of...) |
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caption="1pz5, resolution 1.80Å" /> | caption="1pz5, resolution 1.80Å" /> | ||
'''Structural basis of peptide-carbohydrate mimicry in an antibody combining site'''<br /> | '''Structural basis of peptide-carbohydrate mimicry in an antibody combining site'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PZ5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1PZ5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PZ5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: vaccine design]] | [[Category: vaccine design]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:42:09 2008'' |
Revision as of 14:42, 15 February 2008
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Structural basis of peptide-carbohydrate mimicry in an antibody combining site
Overview
The structure of a complex between the Fab fragment of the antibody, (SYA/J6) specific for the cell surface O-antigen polysaccharide of the, pathogen Shigella flexneri Y and an octapeptide, (Met-Asp-Trp-Asn-Met-His-Ala-Ala), a functional mimic of the O-antigen, has been determined at 1.8-A resolution. Comparison of the structure with, that of the complex with the pentasaccharide antigen, [-->2)-alpha-L-Rha-(1-->2)-alpha-L-Rha-(1-->3)-alpha-L-Rha-(1-->3)-beta-D-, GlcNAc-(1-->2)-alpha-L-Rha-(1-->] reveals the molecular recognition, process by which a peptide mimics a carbohydrate in binding to an, antibody. The binding modes of the two ligands differ considerably., Octapeptide binding complements the shape of the combining site groove, much better than pentasaccharide binding. Moreover, the peptide makes a, much greater number of contacts (126), which are mostly van der Waals, interactions, with the Fab than the saccharide (74). An unusual feature is, also the involvement of 12 water molecules in mediating hydrogen bonds, between residues within the peptide or of the peptide and Fab. Despite, better shape complementarity and greater number of contacts, the, octapeptide binds with an affinity (KA = 2.5 x 10(5) M-1, measured by, calorimetry) only approximately 2-fold tighter than the pentasaccharide., The structural results are relevant to the design of peptide mimetics with, improved affinity for use as vaccines.
About this Structure
1PZ5 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis of peptide-carbohydrate mimicry in an antibody-combining site., Vyas NK, Vyas MN, Chervenak MC, Bundle DR, Pinto BM, Quiocho FA, Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15023-8. Epub 2003 Nov 25. PMID:14645714
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