1q0y
From Proteopedia
(New page: 200px<br /> <applet load="1q0y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q0y, resolution 2.00Å" /> '''Anti-Morphine Antib...) |
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| - | [[Image:1q0y.gif|left|200px]]<br /> | + | [[Image:1q0y.gif|left|200px]]<br /><applet load="1q0y" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1q0y" size=" | + | |
caption="1q0y, resolution 2.00Å" /> | caption="1q0y, resolution 2.00Å" /> | ||
'''Anti-Morphine Antibody 9B1 Complexed with Morphine'''<br /> | '''Anti-Morphine Antibody 9B1 Complexed with Morphine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of an anti-morphine antibody 9B1 (to 1.6A | + | The crystal structures of an anti-morphine antibody 9B1 (to 1.6A resolution) and its complex with morphine (to 2.0 A resolution) are reported. The morphine-binding site is described as a shallow depression on the protein surface, an unusual topology for a high-affinity ( Ka approximately 10(9) M(-1)) antibody against a small antigen. The polar part of the ligand is exposed to solvent, and the cationic nitrogen atom of the morphine molecule is anchored at the bottom of the binding site by a salt-bridge to a glutamate side-chain. Additional affinity is provided by a double cation-pi interaction with two tryptophan residues. Comparison of the morphine complex with the structure of the free Fab shows that a domain closure occurs upon binding of the ligand. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q0Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MOI and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1Q0Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MOI:'>MOI</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pozharski, E.]] | [[Category: Pozharski, E.]] | ||
[[Category: Ringe, D.]] | [[Category: Ringe, D.]] | ||
| - | [[Category: Shanafelt, A | + | [[Category: Shanafelt, A B.]] |
| - | [[Category: Wilson, M | + | [[Category: Wilson, M A.]] |
[[Category: MOI]] | [[Category: MOI]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: fab]] | [[Category: fab]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:40 2008'' |
Revision as of 12:34, 21 February 2008
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Anti-Morphine Antibody 9B1 Complexed with Morphine
Overview
The crystal structures of an anti-morphine antibody 9B1 (to 1.6A resolution) and its complex with morphine (to 2.0 A resolution) are reported. The morphine-binding site is described as a shallow depression on the protein surface, an unusual topology for a high-affinity ( Ka approximately 10(9) M(-1)) antibody against a small antigen. The polar part of the ligand is exposed to solvent, and the cationic nitrogen atom of the morphine molecule is anchored at the bottom of the binding site by a salt-bridge to a glutamate side-chain. Additional affinity is provided by a double cation-pi interaction with two tryptophan residues. Comparison of the morphine complex with the structure of the free Fab shows that a domain closure occurs upon binding of the ligand.
About this Structure
1Q0Y is a Protein complex structure of sequences from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine., Pozharski E, Wilson MA, Hewagama A, Shanafelt AB, Petsko G, Ringe D, J Mol Biol. 2004 Mar 26;337(3):691-7. PMID:15019787
Page seeded by OCA on Thu Feb 21 14:34:40 2008
Categories: Mus musculus | Protein complex | Hewagama, A. | Petsko, G. | Pozharski, E. | Ringe, D. | Shanafelt, A B. | Wilson, M A. | MOI | SO4 | Anti-morphine antibody | Fab
