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2pvd

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[[Image:2pvd.jpg|left|200px]]
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{{STRUCTURE_2pvd| PDB=2pvd | SCENE= }}
{{STRUCTURE_2pvd| PDB=2pvd | SCENE= }}
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'''Crystal srtucture of the reduced ferredoxin:thioredoxin reductase'''
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===Crystal srtucture of the reduced ferredoxin:thioredoxin reductase===
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==Overview==
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Oxygen-evolving photosynthetic organisms regulate carbon metabolism through a light-dependent redox signalling pathway. Electrons are shuttled from photosystem I by means of ferredoxin (Fdx) to ferredoxin-thioredoxin reductase (FTR), which catalyses the two-electron-reduction of chloroplast thioredoxins (Trxs). These modify target enzyme activities by reduction, regulating carbon flow. FTR is unique in its use of a [4Fe-4S] cluster and a proximal disulphide bridge in the conversion of a light signal into a thiol signal. We determined the structures of FTR in both its one- and its two-electron-reduced intermediate states and of four complexes in the pathway, including the ternary Fdx-FTR-Trx complex. Here we show that, in the first complex (Fdx-FTR) of the pathway, the Fdx [2Fe-2S] cluster is positioned suitably for electron transfer to the FTR [4Fe-4S] centre. After the transfer of one electron, an intermediate is formed in which one sulphur atom of the FTR active site is free to attack a disulphide bridge in Trx and the other sulphur atom forms a fifth ligand for an iron atom in the FTR [4Fe-4S] centre--a unique structure in biology. Fdx then delivers a second electron that cleaves the FTR-Trx heterodisulphide bond, which occurs in the Fdx-FTR-Trx complex. In this structure, the redox centres of the three proteins are aligned to maximize the efficiency of electron transfer from the Fdx [2Fe-2S] cluster to the active-site disulphide of Trxs. These results provide a structural framework for understanding the mechanism of disulphide reduction by an iron-sulphur enzyme and describe previously unknown interaction networks for both Fdx and Trx (refs 4-6).
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(as it appears on PubMed at http://www.pubmed.gov), where 17611542 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17611542}}
==About this Structure==
==About this Structure==
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[[Category: Redox]]
[[Category: Redox]]
[[Category: Thioredoxin]]
[[Category: Thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:52:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:14:29 2008''

Revision as of 20:14, 28 July 2008

Image:2pvd.png

Template:STRUCTURE 2pvd

Crystal srtucture of the reduced ferredoxin:thioredoxin reductase

Template:ABSTRACT PUBMED 17611542

About this Structure

2PVD is a Protein complex structure of sequences from Synechocystis sp.. Full crystallographic information is available from OCA.

Reference

Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase., Dai S, Friemann R, Glauser DA, Bourquin F, Manieri W, Schurmann P, Eklund H, Nature. 2007 Jul 5;448(7149):92-6. PMID:17611542

Page seeded by OCA on Mon Jul 28 23:14:29 2008

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