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2pw0

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{{STRUCTURE_2pw0| PDB=2pw0 | SCENE= }}
{{STRUCTURE_2pw0| PDB=2pw0 | SCENE= }}
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'''crystal structure of trans-aconitate bound to methylaconitate isomerase PrpF from Shewanella oneidensis'''
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===crystal structure of trans-aconitate bound to methylaconitate isomerase PrpF from Shewanella oneidensis===
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==Overview==
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In bacteria, the dehydration of 2-methylcitrate to yield 2-methylaconitate in the 2-methylcitric acid cycle is catalyzed by a cofactor-less (PrpD) enzyme or by an aconitase-like (AcnD) enzyme. Bacteria that use AcnD also require the function of the PrpF protein, whose function was previously unknown. To gain insights into the function of PrpF, the three-dimensional crystal structure of the PrpF protein from the bacterium Shewanella oneidensis was solved at 2.0 A resolution. The protein fold of PrpF is strikingly similar to those of the non-PLP-dependent diaminopimelate epimerase from Haemophilus influenzae, a putative proline racemase from Brucella melitensis, and to a recently deposited structure of a hypothetical protein from Pseudomonas aeruginosa. Results from in vitro studies show that PrpF isomerizes trans-aconitate to cis-aconitate. It is proposed that PrpF catalysis of the cis-trans isomerization proceeds through a base-catalyzed proton abstraction coupled with a rotation about C2-C3 bond of 2-methylaconitate, and that residue Lys73 is critical for PrpF function. The newly identified function of PrpF as a non-PLP-dependent isomerase, together with the fact that PrpD-containing bacteria do not require PrpF, suggest that the isomer of 2-methylaconitate that serves as a substrate of aconitase must have the same stereochemistry as that synthesized by PrpD. From this, it follows that the 2-methylaconitate isomer generated by AcnD is not a substrate of aconitase, and that PrpF is required to generate the correct isomer. As a consequence, the isomerase activity of PrpF may now be viewed as an integral part of the 2-methylcitric acid cycle.
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{{ABSTRACT_PUBMED_17567742}}
==About this Structure==
==About this Structure==
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[[Category: Propionate catabolism]]
[[Category: Propionate catabolism]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:48:05 2008''

Revision as of 06:48, 28 July 2008

Image:2pw0.png

Template:STRUCTURE 2pw0

crystal structure of trans-aconitate bound to methylaconitate isomerase PrpF from Shewanella oneidensis

Template:ABSTRACT PUBMED 17567742

About this Structure

2PW0 is a Single protein structure of sequence from Shewanella oneidensis. Full crystallographic information is available from OCA.

Reference

The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function., Garvey GS, Rocco CJ, Escalante-Semerena JC, Rayment I, Protein Sci. 2007 Jul;16(7):1274-84. Epub 2007 Jun 13. PMID:17567742

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