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1sbs
From Proteopedia
(New page: 200px<br /> <applet load="1sbs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sbs, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1sbs.gif|left|200px]]<br /> | + | [[Image:1sbs.gif|left|200px]]<br /><applet load="1sbs" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1sbs" size=" | + | |
caption="1sbs, resolution 2.0Å" /> | caption="1sbs, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF AN ANTI-HCG FAB'''<br /> | '''CRYSTAL STRUCTURE OF AN ANTI-HCG FAB'''<br /> | ||
==Overview== | ==Overview== | ||
| - | 3A2 is an antibody raised against human chorionic gonadotropin and | + | 3A2 is an antibody raised against human chorionic gonadotropin and recognizes a linear epitope on the C-terminal peptide of the human chorionic gonadotropin beta-subunit. Its three-dimensional structure has been determined to 2-A resolution using molecular replacement and refined to a conventional R-factor of 18.2%. The protein exhibits the typical immunoglobulin fold, and the model contains 944 ordered water molecules and one sulfate ion. A comparison of the complementarity-determining regions of the Fab3A2 with those from the Protein Data Bank following the canonical structure method reveals a canonical main chain conformation. This antibody belongs to the canonical structure class (combination of canonical conformations of the complementarity determining loops) that shows a preference for haptens and not for peptides. However, the shape of the surface of the antigen binding loops resembles that of an anti-peptide antibody. |
==About this Structure== | ==About this Structure== | ||
| - | 1SBS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SBS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SBS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Emsley, P.]] | [[Category: Emsley, P.]] | ||
[[Category: Fotinou, C.]] | [[Category: Fotinou, C.]] | ||
| - | [[Category: Isaacs, N | + | [[Category: Isaacs, N W.]] |
| - | [[Category: Schielen, W | + | [[Category: Schielen, W J.G.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: fab-fragment]] | [[Category: fab-fragment]] | ||
| Line 26: | Line 25: | ||
[[Category: reproduction]] | [[Category: reproduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:54 2008'' |
Revision as of 12:59, 21 February 2008
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CRYSTAL STRUCTURE OF AN ANTI-HCG FAB
Overview
3A2 is an antibody raised against human chorionic gonadotropin and recognizes a linear epitope on the C-terminal peptide of the human chorionic gonadotropin beta-subunit. Its three-dimensional structure has been determined to 2-A resolution using molecular replacement and refined to a conventional R-factor of 18.2%. The protein exhibits the typical immunoglobulin fold, and the model contains 944 ordered water molecules and one sulfate ion. A comparison of the complementarity-determining regions of the Fab3A2 with those from the Protein Data Bank following the canonical structure method reveals a canonical main chain conformation. This antibody belongs to the canonical structure class (combination of canonical conformations of the complementarity determining loops) that shows a preference for haptens and not for peptides. However, the shape of the surface of the antigen binding loops resembles that of an anti-peptide antibody.
About this Structure
1SBS is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of an Fab fragment against a C-terminal peptide of hCG at 2.0 A resolution., Fotinou C, Beauchamp J, Emsley P, deHaan A, Schielen WJ, Bos E, Isaacs NW, J Biol Chem. 1998 Aug 28;273(35):22515-8. PMID:9712877
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