2q16

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{{STRUCTURE_2q16| PDB=2q16 | SCENE= }}
{{STRUCTURE_2q16| PDB=2q16 | SCENE= }}
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'''Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP'''
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===Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP===
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==Overview==
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Inosine triphosphate pyrophosphatases, which are ubiquitous house-cleaning enzymes, hydrolyze noncanonical nucleoside triphosphates (inosine triphosphate (ITP) and xanthosine triphosphate (XTP)) and prevent the incorporation of hypoxanthine or xanthine into nascent DNA or RNA. Here we present the 1.5-A-resolution crystal structure of the inosine triphosphate pyrophosphatase RdgB from Escherichia coli in a free state and in complex with a substrate (ITP+Ca(2+)) or a product (inosine monophosphate (IMP)). ITP binding to RdgB induced a large displacement of the alpha1 helix, closing the enzyme active site. This positions the conserved Lys13 close to the bridging oxygen between the alpha- and beta-phosphates of the substrate, weakening the P(alpha)-O bond. On the other side of the substrate, the conserved Asp69 is proposed to act as a base coordinating the catalytic water molecule. Our data provide insight into the molecular mechanisms of the substrate selectivity and catalysis of RdgB and other ITPases.
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{{ABSTRACT_PUBMED_17976651}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Itp pyrophosphatase x-ray structure enzyme mechanism substrate]]
[[Category: Itp pyrophosphatase x-ray structure enzyme mechanism substrate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:09:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:41:52 2008''

Revision as of 02:41, 29 July 2008

Image:2q16.png

Template:STRUCTURE 2q16

Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP

Template:ABSTRACT PUBMED 17976651

About this Structure

2Q16 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular basis of the antimutagenic activity of the house-cleaning inosine triphosphate pyrophosphatase RdgB from Escherichia coli., Savchenko A, Proudfoot M, Skarina T, Singer A, Litvinova O, Sanishvili R, Brown G, Chirgadze N, Yakunin AF, J Mol Biol. 2007 Dec 7;374(4):1091-103. Epub 2007 Oct 11. PMID:17976651

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