From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2q2k.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2q2k.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2q2k| PDB=2q2k | SCENE= }} | | {{STRUCTURE_2q2k| PDB=2q2k | SCENE= }} |
| | | | |
| - | '''Structure of nucleic-acid binding protein'''
| + | ===Structure of nucleic-acid binding protein=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The stable inheritance of genetic material depends on accurate DNA partition. Plasmids serve as tractable model systems to study DNA segregation because they require only a DNA centromere, a centromere-binding protein and a force-generating ATPase. The centromeres of partition (par) systems typically consist of a tandem arrangement of direct repeats. The best-characterized par system contains a centromere-binding protein called ParR and an ATPase called ParM. In the first step of segregation, multiple ParR proteins interact with the centromere repeats to form a large nucleoprotein complex of unknown structure called the segrosome, which binds ParM filaments. pSK41 ParR binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats to mediate both transcription autoregulation and segregation. Here we report the structure of the pSK41 segrosome revealed in the crystal structure of a ParR-DNA complex. In the crystals, the 20-mer tandem repeats stack pseudo-continuously to generate the full-length centromere with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a continuous protein super-helical array, wrapping the DNA about its positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and subsequent plasmid segregation.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18097417}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18097417 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_18097417}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| | [[Category: Protein-dna]] | | [[Category: Protein-dna]] |
| | [[Category: Segregation]] | | [[Category: Segregation]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:12:42 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:51:47 2008'' |
Revision as of 00:51, 28 July 2008
Template:STRUCTURE 2q2k
Structure of nucleic-acid binding protein
Template:ABSTRACT PUBMED 18097417
About this Structure
2Q2K is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Segrosome structure revealed by a complex of ParR with centromere DNA., Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N, Nature. 2007 Dec 20;450(7173):1268-71. PMID:18097417
Page seeded by OCA on Mon Jul 28 03:51:47 2008
