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| | {{STRUCTURE_2q81| PDB=2q81 | SCENE= }} | | {{STRUCTURE_2q81| PDB=2q81 | SCENE= }} |
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| - | '''Crystal Structure of the Miz-1 BTB/POZ domain'''
| + | ===Crystal Structure of the Miz-1 BTB/POZ domain=== |
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| - | ==Overview==
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| - | The POZ/BTB domain is an evolutionarily conserved motif found in approximately 40 zinc-finger transcription factors (POZ-ZF factors). Several POZ-ZF factors are implicated in human cancer, and POZ domain interaction interfaces represent an attractive target for therapeutic intervention. Miz-1 (Myc-interacting zinc-finger protein) is a POZ-ZF factor that regulates DNA-damage-induced cell cycle arrest and plays an important role in human cancer by virtue of its interaction with the c-Myc and BCL6 oncogene products. The Miz-1 POZ domain mediates both self-association and the recruitment of non-POZ partners. POZ-ZF factors generally function as homodimers, although higher-order associations and heteromeric interactions are known to be physiologically important; crucially, the interaction interfaces in such large complexes have not been characterised. We report here the crystal structure of the Miz-1 POZ domain up to 2.1 A resolution. The tetrameric organisation of Miz-1 POZ reveals two types of interaction interface between subunits; an interface of alpha-helices resembles the dimerisation interface of reported POZ domain structures, whereas a novel beta-sheet interface directs the association of two POZ domain dimers. We show that the beta-sheet interface directs the tetramerisation of the Miz-1 POZ domain in solution and therefore represents a newly described candidate interface for the higher-order homo- and hetero-oligomerisation of POZ-ZF proteins in vivo.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17880999}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17880999 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17880999}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Btb/poz domain]] | | [[Category: Btb/poz domain]] |
| | [[Category: Transcription]] | | [[Category: Transcription]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:30:21 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:18:43 2008'' |
Revision as of 02:18, 29 July 2008
Template:STRUCTURE 2q81
Crystal Structure of the Miz-1 BTB/POZ domain
Template:ABSTRACT PUBMED 17880999
About this Structure
2Q81 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A beta-sheet interaction interface directs the tetramerisation of the Miz-1 POZ domain., Stead MA, Trinh CH, Garnett JA, Carr SB, Baron AJ, Edwards TA, Wright SC, J Mol Biol. 2007 Nov 2;373(4):820-6. Epub 2007 Aug 21. PMID:17880999
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