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| | {{STRUCTURE_2q8q| PDB=2q8q | SCENE= }} | | {{STRUCTURE_2q8q| PDB=2q8q | SCENE= }} |
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| - | '''Crystal Structure of S. aureus IsdE complexed with heme'''
| + | ===Crystal Structure of S. aureus IsdE complexed with heme=== |
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| - | ==Overview==
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| - | Staphylococcus aureus is a Gram-positive bacterial pathogen and a leading cause of hospital acquired infections. Because the free iron concentration in the human body is too low to support growth, S. aureus must acquire iron from host sources. Heme iron is the most prevalent iron reservoir in the human body and a predominant source of iron for S. aureus. The iron-regulated surface determinant (Isd) system removes heme from host heme proteins and transfers it to IsdE, the cognate substrate-binding lipoprotein of an ATP-binding cassette transporter, for import and subsequent degradation. Herein, we report the crystal structure of the soluble portion of the IsdE lipoprotein in complex with heme. The structure reveals a bi-lobed topology formed by an N- and C-terminal domain bridged by a single alpha-helix. The structure places IsdE as a member of the helical backbone metal receptor superfamily. A six-coordinate heme molecule is bound in the groove established at the domain interface, and the heme iron is coordinated in a novel fashion for heme transporters by Met(78) and His(229). Both heme propionate groups are secured by H-bonds to IsdE main chain and side chain groups. Of these residues, His(229) is essential for IsdE-mediated heme uptake by S. aureus when growth on heme as a sole iron source is measured. Multiple sequence alignments of homologues from several other Gram-positive bacteria, including the human pathogens pyogenes, Bacillus anthracis, and Listeria monocytogenes, suggest that these other systems function equivalently to S. aureus IsdE with respect to heme binding and transport.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17666394}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17666394 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17666394}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Helical backbone metal receptor superfamily]] | | [[Category: Helical backbone metal receptor superfamily]] |
| | [[Category: Metal transport]] | | [[Category: Metal transport]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:32:47 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:16:24 2008'' |
Revision as of 06:16, 28 July 2008
Template:STRUCTURE 2q8q
Crystal Structure of S. aureus IsdE complexed with heme
Template:ABSTRACT PUBMED 17666394
About this Structure
2Q8Q is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Heme coordination by Staphylococcus aureus IsdE., Grigg JC, Vermeiren CL, Heinrichs DE, Murphy ME, J Biol Chem. 2007 Sep 28;282(39):28815-22. Epub 2007 Jul 31. PMID:17666394
Page seeded by OCA on Mon Jul 28 09:16:24 2008
