2qa9

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{{STRUCTURE_2qa9| PDB=2qa9 | SCENE= }}
{{STRUCTURE_2qa9| PDB=2qa9 | SCENE= }}
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'''Crystal structure of the second tetrahedral intermediates of SGPB at pH 4.2'''
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===Crystal structure of the second tetrahedral intermediates of SGPB at pH 4.2===
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==Overview==
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Streptogrisin B (SGPB) has served as one of the models for studying the catalytic activities of serine peptidases. Here we report its native crystal structures at pH 4.2 at a resolution of 1.18A, and at pH 7.3 at a resolution of 1.23A. Unexpectedly, outstanding electron density peaks occurred in the active site and the substrate-binding region of SGPB in the computed maps at both pHs. The densities at pH 4.2 were assigned as a tetrapeptide, Asp-Ala-Ile-Tyr, whereas those at pH 7.3 were assigned as a tyrosine molecule and a leucine molecule existing at equal occupancies in both of the SGPB molecules in the asymmetric unit. Refinement with relaxed geometric restraints resulted in molecular structures representing mixtures of the second tetrahedral intermediates and the enzyme-product complexes of SGPB existing in a pH-dependent equilibrium. Structural comparisons with the complexes of SGPB with turkey ovomucoid third domain (OMTKY3) and its variants have shown that, upon the formation of the tetrahedral intermediate, residues Glu192A to Gly193 of SGPB move towards the alpha-carboxylate O of residue P1 of the bound species, and adjustments in the side-chain conformational angles of His57 and Ser195 of SGPB favor the progression of the catalytic mechanism of SGPB.
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(as it appears on PubMed at http://www.pubmed.gov), where 18157955 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18157955}}
==About this Structure==
==About this Structure==
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[[Category: Second tetrahedral intermediate]]
[[Category: Second tetrahedral intermediate]]
[[Category: Tetrapeptide]]
[[Category: Tetrapeptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:37:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:26:24 2008''

Revision as of 14:26, 28 July 2008

Image:2qa9.png

Template:STRUCTURE 2qa9

Crystal structure of the second tetrahedral intermediates of SGPB at pH 4.2

Template:ABSTRACT PUBMED 18157955

About this Structure

2QA9 is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

1.2A-resolution crystal structures reveal the second tetrahedral intermediates of streptogrisin B (SGPB)., Lee TW, James MN, Biochim Biophys Acta. 2008 Feb;1784(2):319-34. Epub 2007 Nov 29. PMID:18157955

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