2aab
From Proteopedia
(New page: 200px<br /> <applet load="2aab" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aab, resolution 2.50Å" /> '''Structural basis of...) |
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| - | [[Image:2aab.gif|left|200px]]<br /> | + | [[Image:2aab.gif|left|200px]]<br /><applet load="2aab" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2aab" size=" | + | |
caption="2aab, resolution 2.50Å" /> | caption="2aab, resolution 2.50Å" /> | ||
'''Structural basis of antigen mimicry in a clinically relevant melanoma antigen system'''<br /> | '''Structural basis of antigen mimicry in a clinically relevant melanoma antigen system'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Although mimics of human tumor antigens are effective immunogens to | + | Although mimics of human tumor antigens are effective immunogens to overcome host unresponsiveness to the nominal antigen, the structural basis of this mimicry remains poorly defined. Therefore, in this study we have characterized the structural basis of the human high molecular weight-melanoma-associated antigen (HMW-MAA) mimicry by the mouse anti-idiotypic (anti-id) monoclonal antibody (mAb) MK2-23. Using x-ray crystallography, we have characterized the three-dimensional structure of the anti-id mAb MK2-23 Fab' and shown that its heavy chain complementarity-determining region (CDR3) (H3) and its light chain CDR1 (L1) are closely associated. These moieties are the source of HMW-MAA mimicry, since they display partial amino acid sequence homology along with a similar structural fold with the HMW-MAA core protein. Furthermore, a 15-residue peptide comprising the H3 loop of anti-id mAb MK2-23 demonstrates HMW-MAA-like in vitro and in vivo reactivity. This peptide in conjunction with the structural data will facilitate the characterization of the effect of the degree of antigen mimicry on the induction of a self-antigen-specific immune response by a mimic. |
==About this Structure== | ==About this Structure== | ||
| - | 2AAB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2AAB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chang, C | + | [[Category: Chang, C C.]] |
[[Category: Ferrone, S.]] | [[Category: Ferrone, S.]] | ||
[[Category: Ghosh, D.]] | [[Category: Ghosh, D.]] | ||
| - | [[Category: Hernandez-Guzman, F | + | [[Category: Hernandez-Guzman, F G.]] |
[[Category: Luo, W.]] | [[Category: Luo, W.]] | ||
[[Category: Wang, X.]] | [[Category: Wang, X.]] | ||
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[[Category: molecular mimicry]] | [[Category: molecular mimicry]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:29 2008'' |
Revision as of 14:25, 21 February 2008
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Structural basis of antigen mimicry in a clinically relevant melanoma antigen system
Overview
Although mimics of human tumor antigens are effective immunogens to overcome host unresponsiveness to the nominal antigen, the structural basis of this mimicry remains poorly defined. Therefore, in this study we have characterized the structural basis of the human high molecular weight-melanoma-associated antigen (HMW-MAA) mimicry by the mouse anti-idiotypic (anti-id) monoclonal antibody (mAb) MK2-23. Using x-ray crystallography, we have characterized the three-dimensional structure of the anti-id mAb MK2-23 Fab' and shown that its heavy chain complementarity-determining region (CDR3) (H3) and its light chain CDR1 (L1) are closely associated. These moieties are the source of HMW-MAA mimicry, since they display partial amino acid sequence homology along with a similar structural fold with the HMW-MAA core protein. Furthermore, a 15-residue peptide comprising the H3 loop of anti-id mAb MK2-23 demonstrates HMW-MAA-like in vitro and in vivo reactivity. This peptide in conjunction with the structural data will facilitate the characterization of the effect of the degree of antigen mimicry on the induction of a self-antigen-specific immune response by a mimic.
About this Structure
2AAB is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis of antigen mimicry in a clinically relevant melanoma antigen system., Chang CC, Hernandez-Guzman FG, Luo W, Wang X, Ferrone S, Ghosh D, J Biol Chem. 2005 Dec 16;280(50):41546-52. Epub 2005 Oct 14. PMID:16227204
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