2qhr

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{{STRUCTURE_2qhr| PDB=2qhr | SCENE= }}
{{STRUCTURE_2qhr| PDB=2qhr | SCENE= }}
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'''Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.'''
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===Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.===
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==Overview==
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13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by interactions with the heavy chain. Two GP residues, Gln P406 and Arg P409, make extensive side-chain hydrogen bond and electrostatic interactions with the antibody and are likely critical for recognition and affinity. The 13F6-1-2 antibody utilizes a rare V lambda(x) light chain. The three light-chain complementarity-determining regions do not adopt canonical conformations and represent new classes of structures distinct from V kappa and other V lambda light chains. In addition, although V lambda(x) had been thought to confer specificity, all light-chain contacts are mediated through germ-line-encoded residues. This structure of an antibody that protects against the Ebola virus now provides a framework for humanization and development of a postexposure immunotherapeutic.
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(as it appears on PubMed at http://www.pubmed.gov), where 18005986 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18005986}}
==About this Structure==
==About this Structure==
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[[Category: Immune system/viral protein complex]]
[[Category: Immune system/viral protein complex]]
[[Category: Immunologlobulin fold]]
[[Category: Immunologlobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:59:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 15 10:21:48 2008''

Revision as of 07:21, 15 July 2008

Image:2qhr.png

Template:STRUCTURE 2qhr

Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.

Template:ABSTRACT PUBMED 18005986

About this Structure

Full crystallographic information is available from OCA.

Reference

Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain., Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO, J Mol Biol. 2008 Jan 4;375(1):202-16. Epub 2007 Oct 16. PMID:18005986

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