2qk9

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[[Image:2qk9.gif|left|200px]]
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{{Seed}}
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[[Image:2qk9.png|left|200px]]
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{{STRUCTURE_2qk9| PDB=2qk9 | SCENE= }}
{{STRUCTURE_2qk9| PDB=2qk9 | SCENE= }}
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'''Human RNase H catalytic domain mutant D210N in complex with 18-mer RNA/DNA hybrid'''
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===Human RNase H catalytic domain mutant D210N in complex with 18-mer RNA/DNA hybrid===
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==Overview==
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We report here crystal structures of human RNase H1 complexed with an RNA/DNA substrate. Unlike B. halodurans RNase H1, human RNase H1 has a basic protrusion, which forms a DNA-binding channel and together with the conserved phosphate-binding pocket confers specificity for the B form and 2'-deoxy DNA. The RNA strand is recognized by four consecutive 2'-OH groups and cleaved by a two-metal ion mechanism. Although RNase H1 is overall positively charged, the substrate interface is neutral to acidic in character, which likely contributes to the catalytic specificity. Positions of the scissile phosphate and two catalytic metal ions are interdependent and highly coupled. Modeling of HIV reverse transcriptase (RT) with RNA/DNA in its RNase H active site suggests that the substrate cannot simultaneously occupy the polymerase active site and must undergo a conformational change to toggle between the two catalytic centers. The region that accommodates this conformational change offers a target to develop HIV-specific inhibitors.
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The line below this paragraph, {{ABSTRACT_PUBMED_17964265}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17964265 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17964265}}
==About this Structure==
==About this Structure==
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[[Category: Rna/dna hybrid]]
[[Category: Rna/dna hybrid]]
[[Category: Rnase h]]
[[Category: Rnase h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:06:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:19:42 2008''

Revision as of 15:19, 27 July 2008

Image:2qk9.png

Template:STRUCTURE 2qk9

Human RNase H catalytic domain mutant D210N in complex with 18-mer RNA/DNA hybrid

Template:ABSTRACT PUBMED 17964265

About this Structure

2QK9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription., Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W, Mol Cell. 2007 Oct 26;28(2):264-76. PMID:17964265

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