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2qm4

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{{STRUCTURE_2qm4| PDB=2qm4 | SCENE= }}
{{STRUCTURE_2qm4| PDB=2qm4 | SCENE= }}
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'''Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor'''
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===Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor===
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==Overview==
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The recently characterised 299-residue human XLF/Cernunnos protein plays a crucial role in DNA repair by non-homologous end joining (NHEJ) and interacts with the XRCC4-DNA Ligase IV complex. Here, we report the crystal structure of the XLF (1-233) homodimer at 2.3 A resolution, confirming the predicted structural similarity to XRCC4. The XLF coiled-coil, however, is shorter than that of XRCC4 and undergoes an unexpected reverse in direction giving rise to a short distorted four helical bundle and a C-terminal helical structure wedged between the coiled-coil and head domain. The existence of a dimer as the major species is confirmed by size-exclusion chromatography, analytical ultracentrifugation, small-angle X-ray scattering and other biophysical methods. We show that the XLF structure is not easily compatible with a proposed XRCC4:XLF heterodimer. However, we demonstrate interactions between dimers of XLF and XRCC4 by surface plasmon resonance and analyse these in terms of surface properties, amino-acid conservation and mutations in immunodeficient patients. Our data are most consistent with head-to-head interactions in a 2:2:1 XRCC4:XLF:Ligase IV complex.
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{{ABSTRACT_PUBMED_18046455}}
==About this Structure==
==About this Structure==
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[[Category: Recombination]]
[[Category: Recombination]]
[[Category: Xrcc4 like factor]]
[[Category: Xrcc4 like factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:11:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:43:54 2008''

Revision as of 23:44, 28 July 2008

Image:2qm4.png

Template:STRUCTURE 2qm4

Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor

Template:ABSTRACT PUBMED 18046455

About this Structure

2QM4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human XLF/Cernunnos reveals unexpected differences from XRCC4 with implications for NHEJ., Li Y, Chirgadze DY, Bolanos-Garcia VM, Sibanda BL, Davies OR, Ahnesorg P, Jackson SP, Blundell TL, EMBO J. 2008 Jan 9;27(1):290-300. Epub 2007 Nov 29. PMID:18046455

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