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| - | [[Image:2qm9.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2qm9| PDB=2qm9 | SCENE= }} | | {{STRUCTURE_2qm9| PDB=2qm9 | SCENE= }} |
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| - | '''Troglitazone Bound to Fatty Acid Binding Protein 4'''
| + | ===Troglitazone Bound to Fatty Acid Binding Protein 4=== |
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| - | ==Overview==
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| - | Fatty acid-binding protein 4 (FABP4) delivers ligands from the cytosol to the nuclear receptor PPARgamma in the nucleus, thereby enhancing the transcriptional activity of the receptor. Notably, FABP4 binds multiple ligands with a similar affinity but its nuclear translocation is activated only by specific compounds. To gain insight into the structural features that underlie the ligand-specificity in activation of the nuclear import of FABP4, we solved the crystal structures of the protein complexed with two compounds that induce its nuclear translocation, and compared these to the apo-protein and to FABP4 structures bound to non-activating ligands. Examination of these structures indicates that activation coincides with closure of a portal loop phenylalanine side-chain, contraction of the binding pocket, a subtle shift in a helical domain containing the nuclear localization signal of the protein, and a resultant change in oligomeric state that exposes the nuclear localization signal to the solution. Comparisons of backbone displacements induced by activating ligands with a measure of mobility derived from translation, libration, screw (TLS) refinement, and with a composite of slowest normal modes of the apo state suggest that the helical motion associated with the activation of the protein is part of the repertoire of the equilibrium motions of the apo-protein, i.e. that ligand binding does not induce the activated configuration but serves to stabilize it. Nuclear import of FABP4 can thus be understood in terms of the pre-existing equilibrium hypothesis of ligand binding.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17761196}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17761196 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17761196}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Beta clamshell]] | | [[Category: Beta clamshell]] |
| | [[Category: Lipid binding protein]] | | [[Category: Lipid binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:11:50 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:18:08 2008'' |
Revision as of 10:18, 29 July 2008
Template:STRUCTURE 2qm9
Troglitazone Bound to Fatty Acid Binding Protein 4
Template:ABSTRACT PUBMED 17761196
About this Structure
2QM9 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for activation of fatty acid-binding protein 4., Gillilan RE, Ayers SD, Noy N, J Mol Biol. 2007 Oct 5;372(5):1246-60. Epub 2007 Aug 2. PMID:17761196
Page seeded by OCA on Tue Jul 29 13:18:08 2008
