2qni

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[[Image:2qni.jpg|left|200px]]
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{{Seed}}
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[[Image:2qni.png|left|200px]]
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{{STRUCTURE_2qni| PDB=2qni | SCENE= }}
{{STRUCTURE_2qni| PDB=2qni | SCENE= }}
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'''Crystal structure of uncharacterized protein Atu0299'''
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===Crystal structure of uncharacterized protein Atu0299===
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==Overview==
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 17982461 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17982461}}
==About this Structure==
==About this Structure==
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[[Category: Uncharacterized protein]]
[[Category: Uncharacterized protein]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:15:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:49:41 2008''

Revision as of 12:49, 29 July 2008

Image:2qni.png

Template:STRUCTURE 2qni

Crystal structure of uncharacterized protein Atu0299

Template:ABSTRACT PUBMED 17982461

About this Structure

2QNI is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.

Reference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461

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