This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2qsc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2qsc.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2qsc.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2qsc| PDB=2qsc | SCENE= }}
{{STRUCTURE_2qsc| PDB=2qsc | SCENE= }}
-
'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide'''
+
===Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide===
-
==Overview==
+
<!--
-
F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18068724}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18068724 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18068724}}
==About this Structure==
==About this Structure==
Line 42: Line 46:
[[Category: Viral immunoevasion]]
[[Category: Viral immunoevasion]]
[[Category: Virion]]
[[Category: Virion]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:34:23 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:43:50 2008''

Revision as of 18:43, 28 July 2008

Image:2qsc.png

Template:STRUCTURE 2qsc

Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide

Template:ABSTRACT PUBMED 18068724

About this Structure

2QSC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:18068724

Page seeded by OCA on Mon Jul 28 21:43:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qsc"
Personal tools