2qws

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{{STRUCTURE_2qws| PDB=2qws | SCENE= }}
{{STRUCTURE_2qws| PDB=2qws | SCENE= }}
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'''Neutron and X-ray structural studies of short hydrogen bonds in Photoactive Yellow Protein (PYP)'''
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===Neutron and X-ray structural studies of short hydrogen bonds in Photoactive Yellow Protein (PYP)===
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==Overview==
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Photoactive yellow protein (PYP) from Halorhodospira halophila is a soluble 14 kDa blue-light photoreceptor. It absorbs light via its para-coumaric acid chromophore (pCA), which is covalently attached to Cys69 and is believed to be involved in the negative phototactic response of the organism to blue light. The complete structure (including H atoms) of PYP has been determined in D(2)O-soaked crystals through the application of joint X-ray (1.1 A) and neutron (2.5 A) structure refinement in combination with cross-validated maximum-likelihood simulated annealing. The resulting XN structure reveals that the phenolate O atom of pCA accepts deuterons from Glu46 O(epsilon2) and Tyr42 O(eta) in two unusually short hydrogen bonds. This arrangement is stabilized by the donation of a deuteron from Thr50 O(gamma1) to Tyr42 O(eta). However, the deuteron position between pCA and Tyr42 is only partially occupied. Thus, this atom may also interact with Thr50, possibly being disordered or fluctuating between the two bonds.
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(as it appears on PubMed at http://www.pubmed.gov), where 18007033 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18007033}}
==About this Structure==
==About this Structure==
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[[Category: Sensory transduction]]
[[Category: Sensory transduction]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:42:05 2008''

Revision as of 23:42, 28 July 2008

Template:STRUCTURE 2qws

Neutron and X-ray structural studies of short hydrogen bonds in Photoactive Yellow Protein (PYP)

Template:ABSTRACT PUBMED 18007033

About this Structure

2QWS is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.

Reference

Neutron and X-ray structural studies of short hydrogen bonds in photoactive yellow protein (PYP)., Fisher SZ, Anderson S, Henning R, Moffat K, Langan P, Thiyagarajan P, Schultz AJ, Acta Crystallogr D Biol Crystallogr. 2007 Nov;63(Pt 11):1178-84. Epub 2007, Oct 17. PMID:18007033

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