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| - | [[Image:2qzy.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2qzy.png|left|200px]] |
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| | {{STRUCTURE_2qzy| PDB=2qzy | SCENE= }} | | {{STRUCTURE_2qzy| PDB=2qzy | SCENE= }} |
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| - | '''The structure of chicken mitochondrial PEPCK in complex with PEP'''
| + | ===The structure of chicken mitochondrial PEPCK in complex with PEP=== |
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| - | ==Overview==
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| - | Phosphoenolpyruvate carboxykinase catalyzes the reversible decarboxylation of oxaloacetic acid with the concomitant transfer of the gamma-phosphate of GTP to form PEP and GDP as the first committed step of gluconeogenesis and glyceroneogenesis. The three structures of the mitochondrial isoform of PEPCK reported are complexed with Mn2+, Mn2+-PEP, or Mn2+-malonate-Mn2+ GDP and provide the first observations of the structure of the mitochondrial isoform and insight into the mechanism of catalysis mediated by this enzyme. The structures show the involvement of the hyper-reactive cysteine (C307) in the coordination of the active site Mn2+. Upon formation of the PEPCK-Mn2+-PEP or PEPCK-Mn2+-malonate-Mn2+ GDP complexes, C307 coordination is lost as the P-loop in which it resides adopts a different conformation. The structures suggest that stabilization of the cysteine-coordinated metal geometry holds the enzyme as a catalytically incompetent metal complex and may represent a previously unappreciated mechanism of regulation. A third conformation of the mobile P-loop in the PEPCK-Mn2+-malonate-Mn2+ GDP complex demonstrates the participation of a previously unrecognized, conserved serine residue (S305) in mediating phosphoryl transfer. The ordering of the mobile active site lid in the PEPCK-Mn2+-malonate-Mn2+ GDP complex yields the first observation of this structural feature and provides additional insight into the mechanism of phosphoryl transfer.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16819824}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16819824 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16819824}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pepck]] | | [[Category: Pepck]] |
| | [[Category: Transit peptide]] | | [[Category: Transit peptide]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:59:39 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:56:45 2008'' |
Revision as of 16:56, 28 July 2008
Template:STRUCTURE 2qzy
The structure of chicken mitochondrial PEPCK in complex with PEP
Template:ABSTRACT PUBMED 16819824
About this Structure
2QZY is a Single protein structure of sequence from Gallus gallus. This structure supersedes the now removed PDB entry 2fag. Full crystallographic information is available from OCA.
Reference
Structural insights into the mechanism of PEPCK catalysis., Holyoak T, Sullivan SM, Nowak T, Biochemistry. 2006 Jul 11;45(27):8254-63. PMID:16819824
Page seeded by OCA on Mon Jul 28 19:56:45 2008
