2r3u

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{{STRUCTURE_2r3u| PDB=2r3u | SCENE= }}
{{STRUCTURE_2r3u| PDB=2r3u | SCENE= }}
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'''Crystal structure of the PDZ deletion mutant of DegS'''
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===Crystal structure of the PDZ deletion mutant of DegS===
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==Overview==
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The unfolded protein response of Escherichia coli is triggered by the accumulation of unassembled outer membrane proteins (OMPs) in the cellular envelope. The PDZ-protease DegS recognizes these mislocalized OMPs and initiates a proteolytic cascade that ultimately leads to the sigmaE-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly. The general features of how OMPs activate the protease function of DegS have not yet been systematically addressed. Furthermore, it is unknown how the PDZ domain keeps the protease inactive in the resting state, which is of crucial importance for the functioning of the entire sigmaE stress response. Here we show in atomic detail how DegS is able to integrate the information of distinct stress signals that originate from different OMPs containing a -x-Phe C-terminal motif. A dedicated loop of the protease domain, loop L3, serves as a versatile sensor for allosteric ligands. L3 is capable of interacting differently with ligands but reorients in a conserved manner to activate DegS. Our data also indicate that the PDZ domain directly inhibits protease function in the absence of stress signals by wedging loop L3 in a conformation that ultimately disrupts the proteolytic site. Thus, the PDZ domain and loop L3 of DegS define a novel molecular switch allowing strict regulation of the sigmaE stress response system.
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{{ABSTRACT_PUBMED_17938245}}
==About this Structure==
==About this Structure==
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[[Category: Periplasm]]
[[Category: Periplasm]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:12:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:48:20 2008''

Revision as of 16:48, 28 July 2008

Image:2r3u.png

Template:STRUCTURE 2r3u

Crystal structure of the PDZ deletion mutant of DegS

Template:ABSTRACT PUBMED 17938245

About this Structure

2R3U is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress., Hasselblatt H, Kurzbauer R, Wilken C, Krojer T, Sawa J, Kurt J, Kirk R, Hasenbein S, Ehrmann M, Clausen T, Genes Dev. 2007 Oct 15;21(20):2659-70. PMID:17938245

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