2ri7

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{{STRUCTURE_2ri7| PDB=2ri7 | SCENE= }}
{{STRUCTURE_2ri7| PDB=2ri7 | SCENE= }}
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'''Crystal structure of PHD finger-linker-bromodomain Y17E mutant from human BPTF in the H3(1-9)K4ME2 bound state'''
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===Crystal structure of PHD finger-linker-bromodomain Y17E mutant from human BPTF in the H3(1-9)K4ME2 bound state===
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==Overview==
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Human L3MBTL1, which contains three malignant brain tumor (MBT) repeats, binds monomethylated and dimethylated lysines, but not trimethylated lysines, in several histone sequence contexts. In crystal structures of L3MBTL1 complexes, the monomethyl- and dimethyllysines insert into a narrow and deep cavity of aromatic residue-lined pocket 2, while a proline ring inserts into shallower pocket 1. We have also engineered a single Y to E substitution within the aromatic cage of the BPTF PHD finger, resulting in a reversal of binding preference from trimethyl- to dimethyllysine in an H3K4 sequence context. In both the "cavity insertion" (L3MBTL1) and "surface groove" (PHD finger) modes of methyllysine recognition, a carboxylate group both hydrogen bonds and ion pairs to the methylammonium proton. Our structural and binding studies of these two modules provide insights into the molecular principles governing the decoding of lysine methylation states, thereby highlighting a methylation state-specific layer of histone mark readout impacting on epigenetic regulation.
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{{ABSTRACT_PUBMED_18042461}}
==About this Structure==
==About this Structure==
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[[Category: Zinc finger]]
[[Category: Zinc finger]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
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Revision as of 21:19, 28 July 2008

Image:2ri7.png

Template:STRUCTURE 2ri7

Crystal structure of PHD finger-linker-bromodomain Y17E mutant from human BPTF in the H3(1-9)K4ME2 bound state

Template:ABSTRACT PUBMED 18042461

About this Structure

2RI7 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger., Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ, Mol Cell. 2007 Nov 30;28(4):677-91. PMID:18042461

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