2riq

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{{STRUCTURE_2riq| PDB=2riq | SCENE= }}
{{STRUCTURE_2riq| PDB=2riq | SCENE= }}
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'''Crystal Structure of the Third Zinc-binding domain of human PARP-1'''
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===Crystal Structure of the Third Zinc-binding domain of human PARP-1===
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==Overview==
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Poly(ADP-ribose) polymerase-1 (PARP-1) is a chromatin-associated enzyme with multiple cellular functions, including DNA repair, transcriptional regulation, and cell signaling. PARP-1 has a modular architecture with six independent domains comprising the 113-kDa polypeptide. Two zinc finger domains at the N terminus of PARP-1 bind to DNA and thereby activate the catalytic domain situated at the C terminus of the enzyme. The tight coupling of DNA binding and catalytic activities is critical to the cellular regulation of PARP-1 function; however, the mechanism for coordinating these activities remains an unsolved problem. Here, we demonstrate using spectroscopic and crystallographic analysis that human PARP-1 has a third zinc-binding domain. Biochemical mutagenesis and deletion analysis indicate that this region mediates interdomain contacts important for DNA-dependent enzyme activation. The crystal structure of the third zinc-binding domain reveals a zinc ribbon fold and suggests conserved residues that could form interdomain contacts. The new zinc-binding domain self-associates in the crystal lattice to form a homodimer with a head-totail arrangement. The structure of the homodimer provides a scaffold for assembling the activated state of PARP-1 and suggests a mechanism for coupling the DNA binding and catalytic functions of PARP-1.
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(as it appears on PubMed at http://www.pubmed.gov), where 18055453 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18055453}}
==About this Structure==
==About this Structure==
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[[Category: Zn ribbon]]
[[Category: Zn ribbon]]
[[Category: Zn-binding domain]]
[[Category: Zn-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:59:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:24:56 2008''

Revision as of 04:24, 29 July 2008

Image:2riq.png

Template:STRUCTURE 2riq

Crystal Structure of the Third Zinc-binding domain of human PARP-1

Template:ABSTRACT PUBMED 18055453

About this Structure

2RIQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A Third Zinc-binding Domain of Human Poly(ADP-ribose) Polymerase-1 Coordinates DNA-dependent Enzyme Activation., Langelier MF, Servent KM, Rogers EE, Pascal JM, J Biol Chem. 2008 Feb 15;283(7):4105-14. Epub 2007 Nov 30. PMID:18055453

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