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2ske

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{{STRUCTURE_2ske| PDB=2ske | SCENE= }}
{{STRUCTURE_2ske| PDB=2ske | SCENE= }}
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'''PYRIDOXAL PHOSPHORYLASE B IN COMPLEX WITH PHOSPHITE, GLUCOSE AND INOSINE-5'-MONOPHOSPHATE'''
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===PYRIDOXAL PHOSPHORYLASE B IN COMPLEX WITH PHOSPHITE, GLUCOSE AND INOSINE-5'-MONOPHOSPHATE===
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==Overview==
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It has been established that phosphate analogues can activate glycogen phosphorylase reconstituted with pyridoxal in place of the natural cofactor pyridoxal 5'-phosphate (Change YC. McCalmont T, Graves DJ. 1983. Biochemistry 22:4987-4993). Pyridoxal phosphorylase b has been studied by kinetic, ultracentrifugation, and X-ray crystallographic experiments. In solution, the catalytically active species of pyridoxal phosphorylase b adopts a conformation that is more R-state-like than that of native phosphorylase b, but an inactive dimeric species of the enzyme can be stabilized by activator phosphite in combination with the T-state inhibitor glucose. Co-crystals of pyridoxal phosphorylase b complexed with either phosphite, phosphate, or fluorophosphate, the inhibitor glucose, and the weak activator IMP were grown in space group P4(3)2(1)2, with native-like unit cell dimensions, and the structures of the complexes have been refined to give crystallographic R factors of 18.5-19.2%, for data between 8 and 2.4 A resolution. The anions bind tightly at the catalytic site in a similar but not identical position to that occupied by the cofactor 5'-phosphate group in the native enzyme (phosphorus to phosphorus atoms distance = 1.2 A). The structural results show that the structures of the pyridoxal phosphorylase b-anion-glucose-IMP complexes are overall similar to the glucose complex of native T-state phosphorylase b. Structural comparisons suggest that the bound anions, in the position observed in the crystal, might have a structural role for effective catalysis.
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{{ABSTRACT_PUBMED_8976550}}
==About this Structure==
==About this Structure==
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[[Category: Glycogen phosphorylase]]
[[Category: Glycogen phosphorylase]]
[[Category: Pyridoxal phosphate]]
[[Category: Pyridoxal phosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:34:45 2008''

Revision as of 20:34, 28 July 2008

Image:2ske.png

Template:STRUCTURE 2ske

PYRIDOXAL PHOSPHORYLASE B IN COMPLEX WITH PHOSPHITE, GLUCOSE AND INOSINE-5'-MONOPHOSPHATE

Template:ABSTRACT PUBMED 8976550

About this Structure

2SKE is a Single protein structure of sequence from Oryctolagus cuniculus. This structure supersedes the now removed PDB entry 1ske. Full crystallographic information is available from OCA.

Reference

Activator anion binding site in pyridoxal phosphorylase b: the binding of phosphite, phosphate, and fluorophosphate in the crystal., Oikonomakos NG, Zographos SE, Tsitsanou KE, Johnson LN, Acharya KR, Protein Sci. 1996 Dec;5(12):2416-28. PMID:8976550

Page seeded by OCA on Mon Jul 28 23:34:45 2008

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