2sxl

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{{STRUCTURE_2sxl| PDB=2sxl | SCENE= }}
{{STRUCTURE_2sxl| PDB=2sxl | SCENE= }}
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'''SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE'''
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===SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The Sex-lethal (Sxl) protein from Drosophila melanogaster has two RNA-binding domains (RBDs). As the amino-terminal RBD (RBD1) of the Sxl protein exhibits low sequence homology to the typical RBDs, particularly at the putative functional residues, it was difficult to unambiguously locate the RNP1 and RNP2 motifs. Therefore, in the present study, we defined the amino and carboxy-terminal borders of the first RNA-binding domain (RBD1) of the Sxl protein by limited tryptic digestion. By replacement of Phe166 by Tyr, we constructed a highly soluble mutant, which exhibits the same RNA-binding properties as those of the wild-type. Using this mutant protein, we performed NMR measurements, and elucidated the secondary and tertiary structures of the Sxl RBD1 in solution. The betaalphabetabetaalphabeta folding pattern is conserved in the solution structure of the Sxl RBD1, as in other reported RBD structures. This allowed us to identify both the RNP1 and RNP2 motifs of the Sxl RBD1 unambiguously. Intriguingly, the RNP2 motif of the Sxl RBD1 has an Ile residue at the second position, which is generally occupied by an aromatic amino acid residue in RBDs and has been suggested to be involved in their RNA binding. Furthermore, the loop region between beta2 and beta3 of the Sxl RBD1 has an exceptional cluster of aromatic amino acid residues, in place of the normal basic amino acid cluster. In contrast, the second RBD of Sxl does not exhibit these characteristic features.
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{{ABSTRACT_PUBMED_9299339}}
==About this Structure==
==About this Structure==
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2SXL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SXL OCA].
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2SXL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SXL OCA].
==Reference==
==Reference==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:22:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:33:15 2008''

Revision as of 00:33, 28 July 2008

Image:2sxl.png

Template:STRUCTURE 2sxl

SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9299339

About this Structure

2SXL is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

A characteristic arrangement of aromatic amino acid residues in the solution structure of the amino-terminal RNA-binding domain of Drosophila sex-lethal., Inoue M, Muto Y, Sakamoto H, Kigawa T, Takio K, Shimura Y, Yokoyama S, J Mol Biol. 1997 Sep 12;272(1):82-94. PMID:9299339

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