1a0p
From Proteopedia
(New page: 200px<br /><applet load="1a0p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a0p, resolution 2.5Å" /> '''SITE-SPECIFIC RECOMBI...) |
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| - | [[Image:1a0p.jpg|left|200px]]<br /><applet load="1a0p" size=" | + | [[Image:1a0p.jpg|left|200px]]<br /><applet load="1a0p" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a0p, resolution 2.5Å" /> | caption="1a0p, resolution 2.5Å" /> | ||
'''SITE-SPECIFIC RECOMBINASE, XERD'''<br /> | '''SITE-SPECIFIC RECOMBINASE, XERD'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the site-specific recombinase, XerD, that functions in | + | The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1A0P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1A0P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0P OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arciszewska, L | + | [[Category: Arciszewska, L K.]] |
| - | [[Category: Baker, R | + | [[Category: Baker, R A.]] |
| - | [[Category: Bird, L | + | [[Category: Bird, L E.]] |
| - | [[Category: Sherratt, D | + | [[Category: Sherratt, D J.]] |
| - | [[Category: Subramanya, H | + | [[Category: Subramanya, H S.]] |
| - | [[Category: Wigley, D | + | [[Category: Wigley, D B.]] |
[[Category: dna binding]] | [[Category: dna binding]] | ||
[[Category: dna recombination]] | [[Category: dna recombination]] | ||
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[[Category: xerd]] | [[Category: xerd]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:37 2008'' |
Revision as of 09:39, 21 February 2008
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SITE-SPECIFIC RECOMBINASE, XERD
Overview
The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA.
About this Structure
1A0P is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the site-specific recombinase, XerD., Subramanya HS, Arciszewska LK, Baker RA, Bird LE, Sherratt DJ, Wigley DB, EMBO J. 1997 Sep 1;16(17):5178-87. PMID:9311978
Page seeded by OCA on Thu Feb 21 11:39:37 2008
