2vdf

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{{STRUCTURE_2vdf| PDB=2vdf | SCENE= }}
{{STRUCTURE_2vdf| PDB=2vdf | SCENE= }}
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'''STRUCTURE OF THE OPCA ADHESION FROM NEISSERIA MENINGITIDIS DETERMINED BY CRYSTALLIZATION FROM THE CUBIC MESOPHASE'''
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===STRUCTURE OF THE OPCA ADHESION FROM NEISSERIA MENINGITIDIS DETERMINED BY CRYSTALLIZATION FROM THE CUBIC MESOPHASE===
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==Overview==
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OpcA is an integral outer membrane adhesin protein from Neisseria meningitidis, the causative agent of meningococcal meningitis and septicemia. It binds to sialic acid (SA)-containing polysaccharides on the surface of epithelial cells. The crystal structure of OpcA showed that the protein adopts a 10-stranded beta-barrel structure, with five extensive loop regions on the extracellular side of the membrane. These form a crevice structure, lined with basic residues, which was hypothesized to act as the binding site for polysaccharide ligands. In the current study, a distinctly different OpcA structure has been obtained using crystals grown from a lipidic mesophase. Comparison of the two structures shows that the largest loop (L2), which closes over the end of the beta-barrel in the original crystal form, adopts a much more extended structure by reaching outward and away from the protein. The difference in conformation may be attributable to the absence of zinc ions from the crystallization conditions for the in meso crystal form: in the original structure, two zinc ions were bound to the external loops. Molecular dynamics (MD) simulations performed on the two OpcA models in a lipid bilayer environment demonstrated pronounced loop mobility. These observations support the view that the loop regions of OpcA are capable of a high degree of conformational flexibility. The original binding site for polysaccharide is not present in the in meso crystal form, and is disrupted during MD simulations. Docking analysis suggests a putative alternative location for the SA ligand in the new crystal form of OpcA. Proteins 2008. (c) 2007 Wiley-Liss, Inc.
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(as it appears on PubMed at http://www.pubmed.gov), where 18076035 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18076035}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin., Cherezov V, Liu W, Derrick JP, Luan B, Aksimentiev A, Katritch V, Caffrey M, Proteins. 2007 Dec 12;71(1):24-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18076035 18076035]
In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin., Cherezov V, Liu W, Derrick JP, Luan B, Aksimentiev A, Katritch V, Caffrey M, Proteins. 2007 Dec 12;71(1):24-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18076035 18076035]
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Crystal structure of the OpcA integral membrane adhesin from Neisseria meningitidis., Prince SM, Achtman M, Derrick JP, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3417-21. Epub 2002 Mar 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11891340 11891340]
[[Category: Neisseria meningitidis]]
[[Category: Neisseria meningitidis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Membrane protein]]
[[Category: Membrane protein]]
[[Category: Outer membrane]]
[[Category: Outer membrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:38:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:54:44 2008''

Revision as of 23:54, 27 July 2008

Image:2vdf.png

Template:STRUCTURE 2vdf

STRUCTURE OF THE OPCA ADHESION FROM NEISSERIA MENINGITIDIS DETERMINED BY CRYSTALLIZATION FROM THE CUBIC MESOPHASE

Template:ABSTRACT PUBMED 18076035

About this Structure

2VDF is a Single protein structure of sequence from Neisseria meningitidis. Full crystallographic information is available from OCA.

Reference

In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin., Cherezov V, Liu W, Derrick JP, Luan B, Aksimentiev A, Katritch V, Caffrey M, Proteins. 2007 Dec 12;71(1):24-34. PMID:18076035

Crystal structure of the OpcA integral membrane adhesin from Neisseria meningitidis., Prince SM, Achtman M, Derrick JP, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3417-21. Epub 2002 Mar 12. PMID:11891340

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