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| - | [[Image:2vkx.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2vkx| PDB=2vkx | SCENE= }} | | {{STRUCTURE_2vkx| PDB=2vkx | SCENE= }} |
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| - | '''HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT'''
| + | ===HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT=== |
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| - | ==Overview==
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| - | Activation of the fibroblast growth factor receptor (FGFR) by neural cell adhesion molecule (NCAM) is essential for NCAM-mediated neurite outgrowth. Previous peptide studies have identified two regions in the fibronectin type 3 (FN3)-like domains of NCAM as being important for these activities. Here we report the crystal structure of the NCAM FN3 domain tandem, which reveals an acutely bent domain arrangement. Mutation of a non-conserved surface residue (M610R) led to a second crystal form showing a substantially different conformation. Thus, the FN3 domain linker is highly flexible, suggesting that it corresponds to the hinge seen in electron micrographs of NCAM. The two putative FGFR1-binding segments, one in each NCAM FN3 domain, are situated close to the domain interface. They form a contiguous patch in the more severely bent conformation but become separated upon straightening of the FN3 tandem, suggesting that conformational changes within NCAM may modulate FGFR1 activation. Surface plasmon resonance experiments demonstrated only a very weak interaction between the NCAM FN3 tandem and soluble FGFR1 proteins expressed in mammalian cells (dissociation constant >100 muM). Thus, the NCAM-FGFR1 interaction at the cell surface is likely to depend upon avidity effects due to receptor clustering.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18261743}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18261743 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18261743}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Adhesion receptor]] | | [[Category: Adhesion receptor]] |
| | [[Category: Cell adhesion]] | | [[Category: Cell adhesion]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:58:46 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:49:40 2008'' |
Revision as of 09:49, 29 July 2008
Template:STRUCTURE 2vkx
HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT
Template:ABSTRACT PUBMED 18261743
About this Structure
2VKX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Tandem Fibronectin Type 3 Domains of Neural Cell Adhesion Molecule., Carafoli F, Saffell JL, Hohenester E, J Mol Biol. 2008 Jan 17;. PMID:18261743
Page seeded by OCA on Tue Jul 29 12:49:40 2008
