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2yym

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[[Image:2yym.gif|left|200px]]
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{{STRUCTURE_2yym| PDB=2yym | SCENE= }}
{{STRUCTURE_2yym| PDB=2yym | SCENE= }}
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'''Crystal structure of the mutant of HpaB (T198I, A276G, and R466H) complexed with FAD and 4-hydroxyphenylacetate'''
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===Crystal structure of the mutant of HpaB (T198I, A276G, and R466H) complexed with FAD and 4-hydroxyphenylacetate===
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==Overview==
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The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.
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(as it appears on PubMed at http://www.pubmed.gov), where 17804419 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17804419}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17804419 17804419]
Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17804419 17804419]
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Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Miyatake H, Hisano T, Iwasaki W, Ebihara A, Miki K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt, 7):556-9. Epub 2007 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17620709 17620709]
[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Structurome]]
[[Category: Structurome]]
[[Category: Two-component flavin diffusible monooxygenase]]
[[Category: Two-component flavin diffusible monooxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:42:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:40:51 2008''

Revision as of 13:40, 29 July 2008

Image:2yym.png

Template:STRUCTURE 2yym

Crystal structure of the mutant of HpaB (T198I, A276G, and R466H) complexed with FAD and 4-hydroxyphenylacetate

Template:ABSTRACT PUBMED 17804419

About this Structure

2YYM is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:17804419

Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Miyatake H, Hisano T, Iwasaki W, Ebihara A, Miki K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt, 7):556-9. Epub 2007 Jun 11. PMID:17620709

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