This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2z30

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2z30.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2z30.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2z30| PDB=2z30 | SCENE= }}
{{STRUCTURE_2z30| PDB=2z30 | SCENE= }}
-
'''Crystal structure of complex form between mat-Tk-subtilisin and Tk-propeptide'''
+
===Crystal structure of complex form between mat-Tk-subtilisin and Tk-propeptide===
-
==Overview==
+
<!--
-
Subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-subtilisin) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of the propeptide. The crystal structures of the autoprocessed and mature forms of Tk-subtilisin were determined at 1.89 A and 1.70 A resolution, respectively. Comparison of these structures with that of unautoprocessed Pro-Tk-subtilisin indicates that the structure of Tk-subtilisin is not seriously changed during maturation. However, one unique Ca(2+)-binding site (Ca-7) is identified in these structures. In addition, the N-terminal region of the mature domain (Gly70-Pro82), which binds tightly to the main body in the unautoprocessed form, is disordered and mostly truncated in the autoprocessed and mature forms, respectively. Interestingly, this site is formed also in the unautoprocessed form when its crystals are soaked with 10 mM CaCl(2), as revealed by the 1.87 A structure. Along with the formation of this site, the N-terminal region (Leu75-Thr80) is disordered, with the scissile peptide bond contacting with the active site. These results indicate that the calcium ion binds weakly to the Ca-7 site in the unautoprocessed form, but is trapped upon autoprocessing. We propose that the Ca-7 site is required to promote the autoprocessing reaction by stabilizing the autoprocessed form, in which the new N terminus of the mature domain is structurally disordered. Furthermore, the crystal structure of the Tk-propeptide:S324A-subtilisin complex, which was formed by the addition of separately expressed proteins, was determined at 1.65 A resolution. This structure is virtually identical with that of the autoprocessed form, indicating that the interaction between the two domains is highly intensive and specific.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17706669}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17706669 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17706669}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Subtilisin]]
[[Category: Subtilisin]]
[[Category: Thermococcus kodakaraensis]]
[[Category: Thermococcus kodakaraensis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:53:20 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:52:35 2008''

Revision as of 02:52, 29 July 2008

Image:2z30.png

Template:STRUCTURE 2z30

Crystal structure of complex form between mat-Tk-subtilisin and Tk-propeptide

Template:ABSTRACT PUBMED 17706669

About this Structure

2Z30 is a Protein complex structure of sequences from Thermococcus kodakarensis. Full crystallographic information is available from OCA.

Reference

Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation., Tanaka S, Matsumura H, Koga Y, Takano K, Kanaya S, J Mol Biol. 2007 Sep 28;372(4):1055-69. Epub 2007 Jul 26. PMID:17706669

Page seeded by OCA on Tue Jul 29 05:52:35 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z30"
Personal tools