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3aat

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{{STRUCTURE_3aat| PDB=3aat | SCENE= }}
{{STRUCTURE_3aat| PDB=3aat | SCENE= }}
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'''ACTIVITY AND STRUCTURE OF THE ACTIVE-SITE MUTANTS R386Y AND R386F OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE'''
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===ACTIVITY AND STRUCTURE OF THE ACTIVE-SITE MUTANTS R386Y AND R386F OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE===
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==Overview==
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Arginine-386, the active-site residue of Escherichia coli aspartate aminotransferase (EC 2.6.1.1) that binds the substrate alpha-carboxylate, was replaced with tyrosine and phenylalanine by site-directed mutagenesis. This experiment was undertaken to elucidate the roles of particular enzyme-substrate interactions in triggering the substrate-induced conformational change in the enzyme. The activity and crystal structure of the resulting mutants were examined. The apparent second-order rate constants of both of these mutants are reduced by more than 5 orders of magnitude as compared to that of wild-type enzyme, though R386Y is slightly more active than R386F. The 2.5-A resolution structure of R386F in its native state was determined by using difference Fourier methods. The overall structure is very similar to that of the wild-type enzyme in the open conformation. The position of the Phe-386 side chain, however, appears to shift with respect to that of Arg-386 in the wild-type enzyme and to form new contacts with neighboring residues.
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{{ABSTRACT_PUBMED_1993208}}
==About this Structure==
==About this Structure==
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[[Category: Petsko, G A.]]
[[Category: Petsko, G A.]]
[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:47:23 2008''

Revision as of 01:47, 29 July 2008

Image:3aat.png

Template:STRUCTURE 3aat

ACTIVITY AND STRUCTURE OF THE ACTIVE-SITE MUTANTS R386Y AND R386F OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE

Template:ABSTRACT PUBMED 1993208

About this Structure

3AAT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Activity and structure of the active-site mutants R386Y and R386F of Escherichia coli aspartate aminotransferase., Danishefsky AT, Onnufer JJ, Petsko GA, Ringe D, Biochemistry. 1991 Feb 19;30(7):1980-5. PMID:1993208

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