3b5l

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{{STRUCTURE_3b5l| PDB=3b5l | SCENE= }}
{{STRUCTURE_3b5l| PDB=3b5l | SCENE= }}
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'''Crystal Structure of a Novel Engineered Retroaldolase: RA-61'''
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===Crystal Structure of a Novel Engineered Retroaldolase: RA-61===
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==Overview==
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The creation of enzymes capable of catalyzing any desired chemical reaction is a grand challenge for computational protein design. Using new algorithms that rely on hashing techniques to construct active sites for multistep reactions, we designed retro-aldolases that use four different catalytic motifs to catalyze the breaking of a carbon-carbon bond in a nonnatural substrate. Of the 72 designs that were experimentally characterized, 32, spanning a range of protein folds, had detectable retro-aldolase activity. Designs that used an explicit water molecule to mediate proton shuffling were significantly more successful, with rate accelerations of up to four orders of magnitude and multiple turnovers, than those involving charged side-chain networks. The atomic accuracy of the design process was confirmed by the x-ray crystal structure of active designs embedded in two protein scaffolds, both of which were nearly superimposable on the design model.
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(as it appears on PubMed at http://www.pubmed.gov), where 18323453 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18323453}}
==About this Structure==
==About this Structure==
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[[Category: Retroaldolase]]
[[Category: Retroaldolase]]
[[Category: Xylan degradation]]
[[Category: Xylan degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:24:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:11:59 2008''

Revision as of 16:12, 28 July 2008

Image:3b5l.png

Template:STRUCTURE 3b5l

Crystal Structure of a Novel Engineered Retroaldolase: RA-61

Template:ABSTRACT PUBMED 18323453

About this Structure

3B5L is a Single protein structure of sequence from Thermopolyspora flexuosa. Full crystallographic information is available from OCA.

Reference

De novo computational design of retro-aldol enzymes., Jiang L, Althoff EA, Clemente FR, Doyle L, Rothlisberger D, Zanghellini A, Gallaher JL, Betker JL, Tanaka F, Barbas CF 3rd, Hilvert D, Houk KN, Stoddard BL, Baker D, Science. 2008 Mar 7;319(5868):1387-91. PMID:18323453

Page seeded by OCA on Mon Jul 28 19:11:59 2008

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