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3b9o

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{{STRUCTURE_3b9o| PDB=3b9o | SCENE= }}
{{STRUCTURE_3b9o| PDB=3b9o | SCENE= }}
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'''long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN'''
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===long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN===
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==Overview==
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LadA, a long-chain alkane monooxygenase, utilizes a terminal oxidation pathway for the conversion of long-chain alkanes (up to at least C(36)) to corresponding primary alcohols in thermophilic bacillus Geobacillus thermodenitrificans NG80-2. Here, we report the first structure of the long-chain alkane hydroxylase, LadA, and its complex with the flavin mononucleotide (FMN) coenzyme. LadA is characterized as a new member of the SsuD subfamily of the bacterial luciferase family via a surprising structural relationship. The LadA:FMN binary complex structure and a LadA:FMN:alkane model reveal a hydrophobic cavity that has dual roles: to provide a hydrogen-bond donor (His138) for catalysis and to create a solvent-free environment in which to stabilize the C4a-hydroperoxyflavin intermediate. Consequently, LadA should catalyze the conversion of long-chain alkanes via the acknowledged flavoprotein monooxygenase mechanism. This finding suggests that the ability of LadA to catalyze the degradation of long-chain alkanes is determined by the binding mode of the long-chain alkane substrates. The LadA structure opens a rational perspective to explore and alter the substrate binding site of LadA, with potential biotechnological applications in areas such as petroleum exploration and treatment of environmental oil pollution.
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==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Plasmid]]
[[Category: Plasmid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:15:09 2008''

Revision as of 03:15, 28 July 2008

Image:3b9o.png

Template:STRUCTURE 3b9o

long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN

Template:ABSTRACT PUBMED 18164311

About this Structure

3B9O is a Single protein structure of sequence from Geobacillus thermodenitrificans. Full crystallographic information is available from OCA.

Reference

Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylase., Li L, Liu X, Yang W, Xu F, Wang W, Feng L, Bartlam M, Wang L, Rao Z, J Mol Biol. 2008 Feb 15;376(2):453-65. Epub 2007 Nov 28. PMID:18164311

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