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| | {{STRUCTURE_3bf0| PDB=3bf0 | SCENE= }} | | {{STRUCTURE_3bf0| PDB=3bf0 | SCENE= }} |
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| - | '''Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals'''
| + | ===Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals=== |
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| - | ==Overview==
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| - | Signal peptide peptidase (Spp) is the enzyme responsible for cleaving the remnant signal peptides left behind in the membrane following Sec-dependent protein secretion. Spp activity appears to be present in all cell types, eukaryotic, prokaryotic and archaeal. Here we report the first structure of a signal peptide peptidase, that of the Escherichia coli SppA (SppA(EC)). SppA(EC) forms a tetrameric assembly with a novel bowl-shaped architecture. The bowl has a dramatically hydrophobic interior and contains four separate active sites that utilize a Ser/Lys catalytic dyad mechanism. Our structural analysis of SppA reveals that while in many Gram-negative bacteria as well as characterized plant variants, a tandem duplication in the protein fold creates an intact active site at the interface between the repeated domains, other species, particularly Gram-positive and archaeal organisms, encode half-size, unduplicated SppA variants that could form similar oligomers to their duplicated counterparts, but using an octamer arrangement and with the catalytic residues provided by neighboring monomers. The structure reveals a similarity in the protein fold between the domains in the periplasmic Ser/Lys protease SppA and the monomers seen in the cytoplasmic Ser/His/Asp protease ClpP. We propose that SppA may, in addition to its role in signal peptide hydrolysis, have a role in the quality assurance of periplasmic and membrane-bound proteins, similar to the role that ClpP plays for cytoplasmic proteins.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18164727}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18164727 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18164727}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ser/lys protease]] | | [[Category: Ser/lys protease]] |
| | [[Category: Transmembrane]] | | [[Category: Transmembrane]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:42:17 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:23:26 2008'' |
Revision as of 09:23, 27 July 2008
Template:STRUCTURE 3bf0
Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals
Template:ABSTRACT PUBMED 18164727
About this Structure
3BF0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of a bacterial signal Peptide peptidase., Kim AC, Oliver DC, Paetzel M, J Mol Biol. 2008 Feb 15;376(2):352-66. Epub 2007 Dec 4. PMID:18164727
Page seeded by OCA on Sun Jul 27 12:23:26 2008
