3bfg
From Proteopedia
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{{STRUCTURE_3bfg| PDB=3bfg | SCENE= }} | {{STRUCTURE_3bfg| PDB=3bfg | SCENE= }} | ||
| - | + | ===class A beta-lactamase SED-G238C complexed with meropenem=== | |
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| - | ==Overview== | ||
| - | SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C. | ||
==About this Structure== | ==About this Structure== | ||
| - | 3BFG is a | + | 3BFG is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFG OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:14684905</ref><references group="xtra"/> | |
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Citrobacter sedlakii]] | [[Category: Citrobacter sedlakii]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Pernot, L.]] | [[Category: Pernot, L.]] | ||
[[Category: Petrella, S.]] | [[Category: Petrella, S.]] | ||
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[[Category: Meropenem]] | [[Category: Meropenem]] | ||
[[Category: Sed-g238c]] | [[Category: Sed-g238c]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:07:11 2009'' | ||
Revision as of 13:07, 17 February 2009
class A beta-lactamase SED-G238C complexed with meropenem
About this Structure
3BFG is a 4 chains structure of sequences from Citrobacter sedlakii. Full crystallographic information is available from OCA.
Reference
- Petrella S, Pernot L, Sougakoff W. Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii. Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:14684905
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