3bul

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{{STRUCTURE_3bul| PDB=3bul | SCENE= }}
{{STRUCTURE_3bul| PDB=3bul | SCENE= }}
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'''E. coli I690C/G743C MetH C-terminal fragment (649-1227)'''
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===E. coli I690C/G743C MetH C-terminal fragment (649-1227)===
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==Overview==
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B(12)-dependent methionine synthase (MetH) from Escherichia coli is a large modular protein that is alternately methylated by methyltetrahydrofolate to form methylcobalamin and demethylated by homocysteine to form cob(I)alamin. Major domain rearrangements are required to allow cobalamin to react with three different substrates: homocysteine, methyltetrahydrofolate, and S-adenosyl-l-methionine (AdoMet). These same rearrangements appear to preclude crystallization of the wild-type enzyme. Disulfide cross-linking was used to lock a C-terminal fragment of the enzyme into a unique conformation. Cysteine point mutations were introduced at Ile-690 and Gly-743. These cysteine residues span the cap and the cobalamin-binding module and form a cross-link that reduces the conformational space accessed by the enzyme, facilitating protein crystallization. Here, we describe an x-ray structure of the mutant fragment in the reactivation conformation; this conformation enables the transfer of a methyl group from AdoMet to the cobalamin cofactor. In the structure, the axial ligand to the cobalamin, His-759, dissociates from the cobalamin and forms intermodular contacts with residues in the AdoMet-binding module. This unanticipated intermodular interaction is expected to play a major role in controlling the distribution of conformers required for the catalytic and the reactivation cycles of the enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 18332423 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18332423}}
==About this Structure==
==About this Structure==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Zinc]]
[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:07:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:15:02 2008''

Revision as of 20:15, 27 July 2008

Image:3bul.png

Template:STRUCTURE 3bul

E. coli I690C/G743C MetH C-terminal fragment (649-1227)

Template:ABSTRACT PUBMED 18332423

About this Structure

3BUL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor., Datta S, Koutmos M, Pattridge KA, Ludwig ML, Matthews RG, Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4115-20. Epub 2008 Mar 10. PMID:18332423

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