3rsp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3rsp.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3rsp.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3rsp| PDB=3rsp | SCENE= }}
{{STRUCTURE_3rsp| PDB=3rsp | SCENE= }}
-
'''STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A'''
+
===STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A===
-
==Overview==
+
<!--
-
The peptide bonds preceding Pro 93 and Pro 114 of bovine pancreatic ribonuclease A (RNase A) are in the cis conformation. The trans-to-cis isomerization of these bonds had been indicted as the slow step during protein folding. Here, site-directed mutagenesis was used to replace Pro 93 or Pro 114 with a glycine residue, and the crystalline structure of the P93G variant was determined by X-ray diffraction analysis to a resolution of 1.7 A. This structure is essentially identical to that of the wild-type protein, except for the 91-94 beta-turn containing the substitution. In the wild-type protein, the beta-turn is of type VIa. In the P93G variant, this turn is of type II with the peptide bond preceding Gly 93 being trans. The thermal stabilities of the P93G and P114G variants were assessed by differential scanning calorimetry and thermal denaturation experiments monitored by ultraviolet spectroscopy. The value of delta deltaGm which reports on the stability lost in the variants, is 1.5-fold greater for the P114G variant than for the P93G variant. The greater stability of the P93G variant is likely due to the relatively facile accommodation of residues 91-94 in a type II turn, which has a preference for a glycine residue in its i + 2 position.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9684895}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9684895 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9684895}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Site-directed mutagenesis]]
[[Category: Site-directed mutagenesis]]
[[Category: X-ray diffraction]]
[[Category: X-ray diffraction]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:12:32 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:06:12 2008''

Revision as of 10:06, 3 July 2008

Image:3rsp.png

Template:STRUCTURE 3rsp

STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A

Template:ABSTRACT PUBMED 9684895

About this Structure

3RSP is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structure and stability of the P93G variant of ribonuclease A., Schultz LW, Hargraves SR, Klink TA, Raines RT, Protein Sci. 1998 Jul;7(7):1620-5. PMID:9684895

Page seeded by OCA on Thu Jul 3 13:06:12 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rsp"
Personal tools