429d

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[[Image:429d.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_429d| PDB=429d | SCENE= }}
{{STRUCTURE_429d| PDB=429d | SCENE= }}
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'''CRYSTAL STRUCTURE OF A LEADZYME; METAL BINDING AND IMPLICATIONS FOR CATALYSIS'''
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===CRYSTAL STRUCTURE OF A LEADZYME; METAL BINDING AND IMPLICATIONS FOR CATALYSIS===
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==Overview==
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The leadzyme is a small RNA motif that catalyzes a site-specific, Pb2+-dependent cleavage reaction. As such, it is an example of a metal-dependent RNA enzyme. Here we describe the X-ray crystallographic structure of the leadzyme, which reveals two independent molecules per asymmetric unit. Both molecules feature an internal loop in which a bulged purine base stack twists away from the helical stem. This kinks the backbone, rendering the phosphodiester bond susceptible to cleavage. The independent molecules have different conformations: one leadzyme copy coordinates Mg2+, whereas the other binds only Ba2+ or Pb2+. In the active site of the latter molecule, a single Ba2+ ion coordinates the 2'-OH nucleophile, and appears to mimic the binding of catalytic lead. These observations allow a bond cleavage reaction to be modeled, which reveals the minimal structural features necessary for catalysis by this small ribozyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 10074945 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10074945}}
==About this Structure==
==About this Structure==
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[[Category: Rna]]
[[Category: Rna]]
[[Category: Trna internal loop]]
[[Category: Trna internal loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:18:20 2008''

Revision as of 10:18, 3 July 2008

Image:429d.png

Template:STRUCTURE 429d

CRYSTAL STRUCTURE OF A LEADZYME; METAL BINDING AND IMPLICATIONS FOR CATALYSIS

Template:ABSTRACT PUBMED 10074945

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of a lead-dependent ribozyme revealing metal binding sites relevant to catalysis., Wedekind JE, McKay DB, Nat Struct Biol. 1999 Mar;6(3):261-8. PMID:10074945

Page seeded by OCA on Thu Jul 3 13:18:20 2008

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